PATHWAY PROFILING ANTIBODIES & REAGENTS

     
Products
Product : Antibody >> Antigen Affinity Purified , Neurobiology , New Antibodies , Nuclear & Cytoplasmic.. , Phosphospecific Antibodie.. >> Synapsin I
      
 

Affinity Purified Synapsin I Polyclonal Antibody

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-687
Synapsin IPurified, Peptide Affinity
0.05 mL
 --
 
Catalog No: IMG-687
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 10 ug antibody vial; lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS:137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.  Adequate amount of material to conduct 10 mini Western Blots.
Isotype: Rabbit IgG
Clone: N/A
Purification: Antigen Affinity Chromatography
Species: Human, Mouse, Rat
Host: Rabbit
Application: WB, IHC (cryosections), IP, IF/ICC
Storage: The lyophilized antibody can be stored at -20C and is stable for at least 2 years. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS:137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution the antibody should be aliquot and stored at -20C.
Recommended
Positive Control:
rat brain
 

Background
Synapsin I plays a key role in synaptic plasticity in brain. This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation. Thus synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation.

Antigen
This antibody was developed against Synapsin I native protein purified from bovine brain. The antibody was purified on a Synapsin I affinity column.

Application Notes
In rat brain, a ~78 kDa doublet is observed. Immunolabeling blocked by preadsorption of antibody with Synapsin I. Human, mouse and rat have 100% amino acid sequence for Synapsin I. Antibody dilutions and tissue load should be based on tissue type and expected expression level.

 
Synapsin I Antibody

Western Blot of 10 ug of rat brain lysate showing specific immunolabeling of the ~78 kDa Synapsin I doublet.

-

Reference
1. Stone, L.M., Browning M.D. and Finger T.E., J. Neurosci. 14 (1994) 301 ? 309.
2. Jovanovic, J.N., Sihra, T.S., Nairn, A.C., Hemmings, H.C., Jr., Greengard, P., and Czernik, A.J., J. Neurosci., 21 (2001) 7944 7953.
3. Kao, H.T., Song, H.J., Porton, B., Ming, G.L., Hoh, J., Abraham, M., Czernik, A.J., Pieribone, V.A., Poo, M.M., and Greengard,P., Nature Neurosci., 5 (2002) 431 - 437.
4. Nayak, A.C., Moore, C.I. and Browning, M.D., Proc. Natl. Acad. Sci. (USA) 93 (1996) 15,451 -15,456. 5.Browning, M.D., Dudek, E.M., Rapier, J.L., Leonard, S., and Freedman, R., Biol. Psychiatry 34 (1993) 529 - 535.


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


Products

Services

Resources

Purchasing

Company

  • What's New
  • Reporter Cells
  • Screening Services
  • Interactive Pathways