Antigen Affinity Purified NR2A subunit of the NMDA Receptor Polyclonal Antibody

Background
The ion channels activated by glutamate are typically divided into two classes: those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death. Increased membrane surface expression of the NR2A subunit of the receptor has been associated synaptic plasticity.

Antigen
This polyclonal antibody was raised against C-Terminal fusion protein of the NR2A subunit of the NMDAR. The antibody was purified by affinity chromatography on column made with the C-Terminal fusion protein.

Application Notes
An ~180 kDa band is observed in WB of most brain regions. No reactivity towards the NR2B- or NR2C-Subunit of the NMDA Reactor. Immunolabeling blocked by pre-adsorption of antibody with the C-Terminal fusion protein of the NR2A-Subunit of the NMDA Receptor that was used to generate the antibody. Human, mouse, and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. Antibody dilutions and tissue load should be based on tissue type and expected expression level.

Reference
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