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Datasheet
Peptide-affinity Purified Polyclonal Antibody to G Protein-Coupled Receptor GPR83
Cat.No IMG-71561
Description G Protein-Coupled Receptor GPR83
Format Purified, Peptide Affinity
Unit 0.05 mg
Price(USD) 365.00
MSDS
Catalog No
:
IMG-71561
Contents
:
0.05 mg of purified antibody at a concentration of 1 mg/ml. Phosphate buffered saline, pH 7.7, containing 0.01% sodium azide
Isotype
:
Rabbit IgG
Clone
:
N/A
Purification
:
Antigen Affinity Chromatography
Species
:
Human, Mouse
Host
:
Rabbit
Application
Flow (Cell Surface): Reported to work (Sugimoto N, et al. 2006.) at a dilution of 1:10. Researchers are encouraged to optimize for their model systems IHC (paraffin): 9-18 ug/ml Storage Store at -20°C. For long term storage, store at -70°C
Background GPR83 is a member of the Orphan-A G-Protein coupled receptor family, and has anunknown ligand. It has been previously reported in various regions of the brain, within asubset of T-lymphocytes, and by RT-PCR at low levels in heart, kidney, liver, and otherorgans.
Antigen
Synthetic peptide corresponding to the 3rd extracellular domain of human G Protein-Coupled Receptor GPR83
Application Notes
According to Sugimoto N, et al. 2006., this antibody was demonstrated to work in mouse cells and may be suitable as a cell surface Treg cell marker. It's significance as a marker of human Treg cells has not yet been established.
Flow Cytometric analysis of purified spleen and lymph nodes stained with anti-human GPR83 followed by Alexa488-anti-rabbit IgG. Cells were then stained with APC-anti-CD4 together with PE-anti-CD25 or with PE-anti-FOXP3. Gray is second antibody only and lines are GPR83. Images provided courtesy of Dr. Sakaguchi, Kyoto University, and International Immunology (The Japanese Society for Immunology).
Laser scanning microscopy of fresh prepared (top panel) and activated (bottom panel) cells stained with anti-GPR83, and PE-anti-FOXP3. Red is Foxp3 stained cells, Green is GPR83, and yellow are merged. Images provided courtesy of Dr. Sakaguchi, Kyoto University, and International Immunology (The Japanese Society for Immunology).
Reference 1.G protein-coupled receptor 83 overexpression in naive CD4+CD25- T cells leads to the induction of Foxp3+ regulatory T cells in vivo. Hansen W, Loser K, Westendorf AM, Bruder D, Pfoertner S, Siewert C, Huehn J, Beissert S, Buer J. J Immunol. 2006 Jul 1;177(1):209-15.
Product Citations 1. Foxp3-dependent and -independent molecules specific for CD25+CD4+ natural regulatory T cells revealed by DNA microarray analysis. Sugimoto N, Oida T, Hirota K, Nakamura K, Nomura T, Uchiyama T, Sakaguchi S. Int Immunol. 2006 Aug;18(8):1197-209. Epub 2006 Jun 13. (Flow-Cell surface; Laser scanning microscopy)
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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The protocols described here can be used as a guideline for use of IMGENEX Histo-array slides. Modifications may be necessary depending on the target gene(s), antibodies, or nucleic acid probes.
The protocols described here can be used as a guideline for use of IMGENEX Histo-array slides. Modifications may be necessary depending on the target gene(s), antibodies, or nucleic acid probes.
