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Datasheet
Monoclonal Antibody to Bcl-2 (Clone 124)
Cat.No IMG-80018
Description Bcl-2
Format T.C. Sup.
Unit 0.5 mL
Price(USD) 345.40
MSDS N/A
Catalog No
:
IMG-80018
Contents
:
This antibody is supplied as tissue culture supernatant containing 0.05% sodium azide as preservative.
Isotype
:
Mouse IgG1
Clone
:
124
Species
:
Human
Host
:
Mouse
Application
IHC (frozen):Users should optimize according to model and immunodetection system used (secondary reagents) IHC (paraffin): 1:15-1:50 Western blot analysis: 1:100-750 Storage Store the antibody at 4-8°C. Recommended Positive Control : Human tonsils
Background Principal Names: B-cell CLL/Lymphoma 2 (BCl-2); Apoptosis regulator Bcl-2; Oncogene B-cell leukemia 2 Official Gene Symbol: BCL2 Gen Bank Accession Number: Gene ID: 596 (Human) Gene Map Locus: 18q21.3 (Human) Bcl-2, a founding member of Bcl-2 protein family, is a 25KDa integral membrane protein localized on mitochondria and endoplasmic reticulum. It is a human homolog of C.elegans ced-9 and consists of conserved BH1, BH2, BH3 and BH4 domains and a C-terminal hydrophobic domain. Bcl-2 interacts with various proteins including Bax, RAF1, Myc, BAG1 and NUR77 and is involved in functions including regulating apoptosis, maintaining B-cell memory, regulating calcium signaling and promoting cell growth. The protein also regulates a caspase-activation program independent of Cytochrome-C/Apaf1/caspase-9 apoptosome. Bcl-2 expression is up-regulated by IL-2 and its role as a proto-oncogene has been suggested in follicular lymphoma and tumor angiogenesis.
Antigen
Recombinant bcl-2 peptide sequence comprising of 51-54 amino acids of bcl-2 oncoprotein.
Application Notes
We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. This antibody works well with B5 or Bouin's fixed tissues. Formalin-fixed tissues may give inconsistent results. Specificity: This antibody is specific to bcl-2 oncoprotein, encoded by agene involved in the 14; 18 chromosomal translocation. In lymphoid tissues, this antibody stains B-lymphocytes as well as T cells. Cellular Localization: Cell membrane.
IHC analysis of Bcl-2 in formalin fixed paraffin-embedded human tonsils using IMG-80018.
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Reference 1. Tsujimoto et al. Proc Natl Acad Sci (USA) 83: 5214, 1986. 2. Clearly et al. cell 47: 19, 1986. 3. Hockenbery et al. Nature 348: 334, 1990. 4. Swanson et at. Applied Immunohistochemistry 1: 182, 1993. 5. Nunez et.al Nature 353(6339):71-3 (1991 Sep 5) 6. Wang et.al Cell. 87(4):629-38 (1996 Nov 15) 7. Karl et.al Cancer Research 65, 5063-5069 (June 15, 2005)
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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IMG-80018 [Monoclonal Antibody to Bcl-2 (Clone 124)]
