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Product : Antibody >> Cancer Antibodies , CD Markers , Innate Immunology , Stem Cell Markers >> CD34
      
 

Monoclonal Antibody to CD34 (Clone QBEND/10)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-80073
CD34Purified
0.5 mL
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Catalog No: IMG-80073
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: This antibody is supplied as purified immunoglobulin containing 0.05% sodium azide as preservative.
Isotype: Mouse IgG1
Clone: QBEND/10
Species: Human
Host: Mouse
Application: IHC (p): 1:25-1:100
Storage: Store the antibody at 4-8°C.
Recommended
Positive Control:
Human T cell line
 

Background
Principal Names: Hematopoietic Progenitor cell antigen; CD34 antigen; Official Gene Symbol: CD34 Gene ID: 947 (Human) Gene Map Locus: 1q32 (Human)CD34, an 110KDa monomeric cell surface glycoprotein, is a member of Sialomucin family. It is a Type I transmembrane protein selectively expressed on surface of human hematopoietic progenitor cells and endothelial cells. It consists of a heavily glycosylated extracellular domain, a transmembrane region and an intracellular cytoplasmic domain containing potential phosphorylation sites. Physiological functions of CD34 antigen include role in early hematopoiesis, cell-cell adhesion, and binding of L-selectin in high endothelial venules. CD34 antigen is frequently used for positive selection of hematopoietic stem/progenitor cells in research and transplantation. Pathological role of CD34+ stromal cells has been suggested in ALL and lung adenocarcinomas.

Antigen
Endothelial cells (HUVE).

Application Notes
Monoclonal antibodies to CD34 can be classified into three main classes, class I, class II and class III, on the basis of differential sensitivity to corresponding CD34 epitopes to degradation by specific enzymes. The IMG-80073 is a class II monoclonal antibody which recognizes a CD34 epitope resistant to neuraminidase, and sensitive to glycoprotease and chymopapain.  We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Specificity: This antibody reacts with a glycoprotein of MW 110 kD, present on the surface of hematopoietic cells i.e. myeloid and lymphoid progenitors. It stains the vascular endothelial cells. Cellular Localization: Cell membrane.

Genebank Info (Nucleotide): NP_001764

 
CD34 Antibody

Western blot analysis of CD34 using IMG-80073 in human T cell line extract.

CD34 Antibody IMG-80073

IHC analysis of CD34, Endothelial Cell formalin-fixed paraffin embedded human tonsil using IMG-80073.

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Reference
1. Anthony et al. J Clin Pathol 44: 29, 1991.
2. Fina et al. Blood 75: 2417, 1990.
3. Ramani et al. Histopathol 17: 237, 1990.
4. Lanza et.al Journal of Biological Regulators and Homeostatic Agents 15 (1) (2001)
5. Fina et.al Blood. 75(12):2417-26 (1990 Jun 15)
6. Serke S, van Lessen A, Huhn D. Quantitative fluorescence flow cytometry: a comparison of the three techniques for direct and indirect immunofluorescence. Cytometry 1998; 33:179-87.


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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