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Datasheet
Monoclonal Antibody to Androgen Receptor (Clone AR 441)
Cat.No IMG-80164
Description Androgen Receptor
Unit 0.5 mL
Price(USD) 345.40
MSDS N/A
Catalog No
:
IMG-80164
Contents
:
This antibody is supplied as purified immunoglobulin fraction containing 0.05% sodium azide as preservative.
Isotype
:
Mouse IgG1
Clone
:
AR 441
Species
:
Human
Host
:
Mouse
Application
Western blot analysis: 1:50 - 1:100 IHC (frozen):Users should optimize according to model and immunodetection system used IHC (paraffin): 1:25-1:50 Storage Store the antibody at 4-8°C. Recommended Positive Control : Prostate carcinoma
Background
Principal Names: Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease) Official Gene Symbol: AR Gene ID: 367 (Human) Gene Map Locus: Xq11.2-q12 (Human) Androgen Receptor is a member of the steroid/nuclear receptor protein superfamily, functioning as a steroid-hormone (androgen) activated transcription factor. The protein has four major functional regions: the N-terminal transactivation domain (TAD), a central DNA-binding domain (DBD), a C-terminal ligand-binding domain (LBD), and a hinge region connecting the DBD and LBD. The receptor upon binding with the ligand, androgen dihydrotestosterone, dissociates from accessory proteins, translocates into the nucleus, dimerizes, and binds to the androgen-response elements, thereby activating genes involved in cell growth and proliferation. The transcriptional activity of AR is affected by coregulators like members of the p160 family, ARA70, ARA55, ARA54, ARA267-á, Smad-3, and AIB1 which influence a number of functional properties of AR, including ligand selectivity and DNA binding capacity. The gene is located in the Xq11.2–q12 chromosome and consists of eight exons. The pathological role of the protein has been observed in metastatic prostatrate cancer, infertility male syndrome, Reifenstein syndrome and androgen insensibility syndrome.
Antigen
BALB/C mice were injected with a synthetic peptide, amino acid 299-315 (STEDTAEYSPFKGGYTK) of human androgen receptor.
Application Notes
We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Specificity: This antibody is specific to a protein of 110 kD, identified as androgen receptor. This antibody reacts with full length of AR and also with the newly described A form of the receptor. This antibody does not cross react with estrogen, progesterone or glucocorticoid receptors. Cellular Localization: Nuclear.
IHC analysis of Androgen Receptor in formalin-fixed paraffin embedded human prostate using IMG-80164.
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Related Products
Reference 1. New England Journal of Medicine 351; 15, 2004.
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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IMG-80164 [Monoclonal Antibody to Androgen Receptor (Clone AR 441)]
