Background
The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed proteins. All of the Hsp70 proteins have three major functional domains. An N-terminal ATPase domain binds ATP (Adenosine triphosphate) and hydrolyzes it to ADP (Adenosine diphosphate), a substrate binding domain contains a groove with an affinity for neutral, hydrophobic amino acid residues and a C-terminal domain rich in alpha helical structure acts as a 'lid' for the substrate binding domain. Eukaryotic organisms express several slightly different Hsp70 proteins. Hsc70 or Hsp73; a constitutively expressed chaperone protein, Hsp70 or Hsp72; a stress-induced protein, BiP or Grp78; a protein localized to the endoplasmic reticulum mtHsp70 or Grp75; the mitochondrial Hsp70. Heat shock protein 72 (Hsp72) is the major inducible, 70kDa heat shock protein. It plays a role in many cellular activities including protein synthesis, folding, and translocation into organelles as well as the assembly of multiprotein complexes. Hsc70 typically makes up one to three percent of total cellular protein. The genes are located in the major histocompatibility complex class III region. |
Antigen
BALB/C mice were injected with purified HSP70 protein. |
Application Notes
We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Specificity: This antibody is specific to constitutive (HSP73) and inducible (HSP72) form of HSP70. HSP70 is found to be associated with steroid receptors, actin, p53, polyoma T antigen, nucleotides and other known proteins. HSP70 has been shown to be involved in protective role against thermal stress, cytotoxic drugs and other damaging conditions. Cellular Localization: Cytoplasmic and nuclear. |
Genebank Info (Protein)
NP_002145 |
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IHC analysis of Heat Shock Protein 70 formalin-fixed paraffin embedded human breast carcinoma using IMG-80181. |
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Reference
1. Yoshimura et al. Biochem Biophys Res Commun 293: 1389, 2002.
2. Wegele et al. Rev Physiol Biochem Pharmacol 151:1, 2004.
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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