Monoclonal Antibody to TAG-72 (Clone CC49)

Background
Tumor-associated glycoprotein (TAG) 72 is a tumor marker, identified and characterized using two different monoclonal antibodies B72.3 and CC49. It is a mucin-like protein of high molecular weight (220-400 kd). The protein is expressed in several epithelial-derived cancers including 94% of colonic adenocarcinomas, 84% of invasive ductal carcinomas of the breast, 96% of non-small cell lung carcinomas, 100%of common epithelial ovarian carcinomas, as well as the majority of pancreatic, gastric, and esophageal cancers evaluated. The protein expression is also detected in fetal colon, stomach, and esophagus, thus defining TAG-72 as an oncofetal antigen. The protein is pancarcinoma in nature. The pancarcinoma distribution and lack of significant reactivity with normal tissues of monoclonal antibody B72.3 suggests that Tag-72 has thus a potential diagnostic and therapeutic utility for human carcinomas.

Antigen
BALB/C mice were injected with purified TAG-72 protein.

Application Notes
We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Specificity: This antibody is specific to an oncofetal antigen of >1,000 kD known as tumor associated glycoprotein (TAG-72) or CA 72-4. This antibody has been shown to be distinct from B72.3 by reciprocal competition radio-immunoassays and it has a Ka higher than that of B72.3. TAG-72 is expressed in colon, breast, pancreatic, ovarian, lung and gastric cancer. Cellular Localization: Cytoplasm, cell surface.

Reference
1. Colcher et al. Can Res 48: 4597, 1988.
2. Katari et al. Can Res 50: 4885, 1990.
3. Sheer et al. Can Res 48: 6811, 1988.
4. Filella et al. Anticancer Res 20: 5229, 2000.
5. Negishi et al. Gynecol Oncol. 48:148, 1993.
6. Thor et al. Cancer Research 46; 3118 1986.