We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. High temperature treatment of formalin-fixed tissue sections with 10mM citrate buffer, pH 6.0 must be performed prior to the immunostaining. Specificity: This antibody reacts with a 16.5 kD protein, known as Survivin. Survivin is an inhibitor of apoptosis protein. It is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle. Disruption of survivin-microtubule interactions results in loss of survivin’s antiapoptosis function and increased Caspase-3 activity. This antibody cross reacts with human and mouse. Cellular Localization: Cytoplasmic.