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Peptide-affinity Purified Polyclonal Antibody to ATF4 (Ser245) |
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| Catalog No: |
IMG-90166-1 |
| Contents: |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. |
| Species: |
Human |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 1:50-1:100
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| Storage: |
Store at -20°C. |
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Background
ATF4 encodes a cAMP response element (CRE) binding protein called CREB-2 which acts as a transcriptional activator. The protein contains a COOH-terminal leucine-zipper motif and an adjacent basic domain. The protein is expressed ubiquitously in human brain and umbilical vein endothelial cell. The protein binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. The protein is localized in the 22q13.1 region of human chromosome. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication. ATF4 negatively regulate transcription from the cAMP response element. |
Antigen
This antibody was generated by immunizing rabbits with a synthetic phosphopeptide corresponding to human ATF4 around the phosphorylation site of serine 245 (N-R-SP-L-P). |
| Genebank Info (Protein): NP_001666 |
| Gene ID: 468 |
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Immunohistochemical analysis of ATF4 (Ser245) in paraffin-embedded human breast carcinoma tissue using IMG-90166-1. |
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Reference
1. Karpinski, BA. et al. Proc. Natl. Acad. Sci. U S A. 89:4820-4 (1992).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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