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Peptide-affinity Purified Polyclonal Antibody to cdc25C (Ser216) |
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| Catalog No: |
IMG-90219-1 |
| Contents: |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. |
| Species: |
Human |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 1:50-1:100 |
| Storage: |
Store at -20°C. |
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Background
CDC25C is a member of Cdc25 phosphatase family which plays prominent role in the regulation of eukaryotic cell cycle progression. CDC25C activates the mitotic-promoting factor cyclin B1/cdc2 by dephosphorylating T14 and Y15 of cdc2/cyclin B and thus triggers G2/M transition. The protein acts as a converging point of several signal transduction cascades and links the mitotic activation of cdc2/cyclin B to the DNA replication and repair checkpoint. The enzymatic activity of CDC25C is low during interphase and increases sharply at the G2ƒ}M transition point. The protein is nuclear throughout interphase. Alternate splicing of its mRNA results in multiple transcript variants. Human CDC25C gene is localized in the chromosomal region 5q31. |
Antigen
This antibody was generated by immunizing rabbits with a synthetic phosphopeptide corresponding to human cdc25C around the phosphorylation site of serine 216 (S-P-SP-M-P). |
Genebank Info (Protein)
NP_073720 |
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Immunohistochemical analysis of cdc25C (Ser216) in paraffin-embedded human breast carcinoma tissue using IMG-90219-1. |
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Reference
1. Sartor, H. et al. Genomics. 13:911-912 (1992).
2. Zwicker, J. et al. Prog. Cell. Cycle. 1:91-99 (1995).
3. Schwindling, SL. et al. Nature. 23:4155-4165 (2004).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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