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Product : Antibody >> Cell Cycle , Kinases & Phosphatase.. , Phosphospecific Antibodie.. >> cdc25C (Ser216)
      
 

Peptide-affinity Purified Polyclonal Antibody to cdc25C (Ser216)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-90219-1
cdc25C (Ser216)Purified, Peptide Affinity
50 µg
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Catalog No: IMG-90219-1
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Isotype: Rabbit IgG
Clone: N/A
Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns.
Species: Human
Host: Rabbit
Application: IHC (paraffin): 1:50-1:100
Storage: Store at -20C.
 

Background
CDC25C is a member of Cdc25 phosphatase family which plays prominent role in the regulation of eukaryotic cell cycle progression. CDC25C activates the mitotic-promoting factor cyclin B1/cdc2 by dephosphorylating T14 and Y15 of cdc2/cyclin B and thus triggers G2/M transition. The protein acts as a converging point of several signal transduction cascades and links the mitotic activation of cdc2/cyclin B to the DNA replication and repair checkpoint. The enzymatic activity of CDC25C is low during interphase and increases sharply at the G2}M transition point. The protein is nuclear throughout interphase. Alternate splicing of its mRNA results in multiple transcript variants. Human CDC25C gene is localized in the chromosomal region 5q31.

Antigen
This antibody was generated by immunizing rabbits with a synthetic phosphopeptide corresponding to human cdc25C around the phosphorylation site of serine 216 (S-P-SP-M-P).

Genebank Info (Protein): NP_073720
Gene ID: 995
SwissProt: P30307
 
cdc25C (Ser216) Antibody IMG-90219-1

Immunohistochemical analysis of cdc25C (Ser216) in paraffin-embedded human breast carcinoma tissue using IMG-90219-1.

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Reference
1. Sartor, H. et al. Genomics. 13:911-912 (1992).
2. Zwicker, J. et al. Prog. Cell. Cycle. 1:91-99 (1995).
3. Schwindling, SL. et al. Nature. 23:4155-4165 (2004).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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