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Peptide-affinity Purified Polyclonal Antibody to cdc25A (Ser75) |
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| Catalog No: |
IMG-90236-1 |
| Contents: |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. |
| Species: |
Human, Mouse, Rat |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 1:50-1:100
Western Blot: 1:500-1:1000 |
| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
A2780 cells |
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Background
cdc25A belongs to the Cdc25 family phosphatases which are dual-specificity phosphatases, and can dephosphorylate phosphotyrosine as well as phosphothreonine residues, therefore activating their physiological substrates, the cyclin-dependent protein kinases (Cdks) and thus have specific roles in cell-cycle regulation. cdc25A has a comprehensive function in the cell cycle, regulating the G1/S transition, S phase and the G2/M transition. Its inactivation can contribute to an immediate block of the cell cycle, leading to an apparently p53-independent G1 arrest, an S-phase delay or a G2 arrest. In human cells, cdc25A is localized to the nucleus. Accelerated Cdc25A degradation, as a result of phosphorylation by the Chk1 and/or Chk2 protein kinases, results in sustained inhibitory phosphorylation of Cdk2, leading to G1 arrest and a block in S-phase entry. It often acts as an oncogene. |
Antigen
This antibody was generated by immunizing rabbits with a synthetic phosphopeptide corresponding to human cdc25A around the phosphorylation site of serine 75 (M-G-SP-S-E). |
Genebank Info (Protein)
NP_963861 |
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Western blot analysis of cdc25A (Ser75) (Lanes 3 and 4) using IMG-90236-1 in A2780 cells. |
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Immunohistochemical analysis of cdc25A (Ser75) in paraffin-embedded human breast carcinoma tissue using IMG-90236-1. |
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Reference
1. Draetta, J. et al. J. Biochem. Biophys. Acta. 1332:M53-M63 (1997).
2. Gabrielli, BG. et al. Oncogene. 15:749-758 (1997).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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