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Product : Antibody >> Cell Cycle , Kinases & Phosphatase.. , Phosphospecific Antibodie.. >> cdc25A (Ser75)
      
 

Peptide-affinity Purified Polyclonal Antibody to cdc25A (Ser75)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-90236-1
cdc25A (Ser75)Purified, Peptide Affinity
50 µg
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Catalog No: IMG-90236-1
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Isotype: Rabbit IgG
Clone: N/A
Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns.
Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC (paraffin): 1:50-1:100
Western Blot: 1:500-1:1000
Storage: Store at -20C.
Recommended
Positive Control:
A2780 cells
 

Background
cdc25A belongs to the Cdc25 family phosphatases which are dual-specificity phosphatases, and can dephosphorylate phosphotyrosine as well as phosphothreonine residues, therefore activating their physiological substrates, the cyclin-dependent protein kinases (Cdks) and thus have specific roles in cell-cycle regulation. cdc25A has a comprehensive function in the cell cycle, regulating the G1/S transition, S phase and the G2/M transition. Its inactivation can contribute to an immediate block of the cell cycle, leading to an apparently p53-independent G1 arrest, an S-phase delay or a G2 arrest. In human cells, cdc25A is localized to the nucleus. Accelerated Cdc25A degradation, as a result of phosphorylation by the Chk1 and/or Chk2 protein kinases, results in sustained inhibitory phosphorylation of Cdk2, leading to G1 arrest and a block in S-phase entry. It often acts as an oncogene.

Antigen
This antibody was generated by immunizing rabbits with a synthetic phosphopeptide corresponding to human cdc25A around the phosphorylation site of serine 75 (M-G-SP-S-E).

Genebank Info (Protein): NP_963861
Gene ID: 993
SwissProt: P30304
 
cdc25A (Ser75) Antibody

Western blot analysis of cdc25A (Ser75) (Lanes 3 and 4) using IMG-90236-1 in A2780 cells.

cdc25A (Ser75) Antibody IMG-90236-1

Immunohistochemical analysis of cdc25A (Ser75) in paraffin-embedded human breast carcinoma tissue using IMG-90236-1.

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Reference
1. Draetta, J. et al. J. Biochem. Biophys. Acta. 1332:M53-M63 (1997).
2. Gabrielli, BG. et al. Oncogene. 15:749-758 (1997).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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