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Product : Antibody >> Cell Cycle , Kinases & Phosphatase.. >> Chk2 (Ser516)
      
 

Peptide-affinity Purified Polyclonal Antibody to Chk2 (Ser516)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-90243-1
Chk2 (Ser516)Purified, Peptide Affinity
50 µg
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Catalog No: IMG-90243-1
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Isotype: Rabbit IgG
Clone: N/A
Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns.
Species: Human
Host: Rabbit
Application: Western Blot: 1:500-1:1000
Storage: Store at -20C.
Recommended
Positive Control:
HeLa cells
 

Background
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer.

Antigen
This antibody was generated by immunizing rabbits with a synthetic phosphopeptide corresponding to human Chk2 around the phosphorylation site of serine 516 (Q-P-SP-T-S).

Genebank Info (Protein): NP_009125
Gene ID: 11200
SwissProt: O96017
 
Chk2 (Ser516) Antibody

Western blot analysis of Chk2 (phospho-Ser516) using IMG-90243-1 in HeLa cells.

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Reference
1. Lee, JS. et al. Nature. 404:201-214 (2000).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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