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Peptide-affinity Purified Polyclonal Antibody to EGFR (Thr693) |
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| Catalog No: |
IMG-90273-1 |
| Contents: |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. |
| Species: |
Human, Mouse, Rat |
| Host: |
Rabbit |
| Application: |
Western Blot: 1:500-1:1000 |
| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
A431 cells |
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Background
EGFR is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Besides EGF, the protein is also a receptor for other proteins like amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. The protein is involved in the control of cell growth and differentiation. The protein is usually expressed in placenta. Mutations affecting EGFR expression or activity could result in cancer. Human EGFR gene is mapped to chromosome 7p12. |
Antigen
This antibody was generated by immunizing rabbits with a synthetic phosphopeptide corresponding to human EGFR around the phosphorylation site of threonine 693 (P-L-TP-P-S). |
Genebank Info (Protein)
NP_005219 |
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Western blot analysis of EGFR (Thr693) using IMG-90273-1 A431 cells untreated or treated with EGF (200ng/ml, 5min). |
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Reference
1. Haley, J. et al. et al. Oncogene. Res. 1:375-396 (1987).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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