|
|
|
| |
Peptide-affinity Purified Polyclonal Antibody to 4E-BP1 (Thr45) |
|
|
| |
|
|
| |
|
| Catalog No: |
IMG-90293-1 |
| Contents: |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. |
| Species: |
Human, Mouse, Rat |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 1:50-1:100
Western Blot: 1:500-1:1000 |
| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
MDA435 cells |
|
 |
|
|
|
|
|
|
Background
The translation of mRNA in eukaryotic cells is regulated by the presence of amino acids through multiple mechanisms. One such mechanism involves the evolutionarily conserved serine/threonine kinase TOR (Target of rapamycin, also known as mTOR), which regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. One downstream target of TOR is the eukaryotic initiation factor 4E binding protein 1 (4E-BP1) whose phosphorylation prevents its association with eIF4E, preferentially stimulating translation of mRNAs containing long, highly structured 5’-UTRs. Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation, at least in part by inhibiting the activity of TOR towards 4E-BP1. |
Antigen
This antibody was generated by immunizing rabbits with a synthetic phosphopeptide corresponding to human 4E-BP1 around the phosphorylation site of threonine 45 (S-T-TP-P-G). |
Genebank Info (Protein)
NP_004086 |
| |
|
Western blot analysis of 4E-BP1 (Thr45) using IMG-90293-1 in MDA435 cells untreated or treated with EGF (200nm, 5min). |
|
|
|
|
Immunohistochemical analysis of 4E-BP1 (Thr45) in paraffin-embedded human breast carcinoma tissue using IMG-90293-1. |
|
|
|
|
|
| - |
 |
|
Reference
1. Fingar, DC. et al. Oncogene. 23:3151-3171 (2004).
2. Shamji, AF. et al. Mol. Cell. 12:271-280 (2003).
|
|
|
|
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
|
|
|
|
|
