|
|
|
| |
Peptide-affinity Purified Polyclonal Antibody to IRS-1 (Ser307) |
|
|
| |
|
|
| |
|
| Catalog No: |
IMG-90304-1 |
| Contents: |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. |
| Species: |
Human, Mouse, Rat |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 1:50-1:100 |
| Storage: |
Store at -20°C. |
|
 |
|
|
|
|
|
|
Background
IRS-1 regulates most insulin responses, including peripheral and central glucose homeostasis, protein synthesis, and cell proliferation and growth. The protein contains an N-terminal pleckstrin homology (PH) domain and phosphotyrosine-binding (PTB) domain which are necessary for targeting IRS-1 to the activated insulin receptor at plasma membrane, where it becomes phosphorylated in response to insulin stimulation of cells. Once phosphorlated by the insulin receptor, the protein binds specifically to various cellular proteins containing Src homology-2 (Sh2) domain, including phosphatidylinositol 3-kinase (PI-3K), Grb2-Sos, and protein tyrosine phosphatase type 11 (PTPN11 or SHP2). In humans, the pathological role of the protein has been detected in non-insulin-dependent diabetes mellitus. |
Antigen
This antibody was generated by immunizing rabbits with a synthetic phosphopeptide corresponding to human IRS-1 around the phosphorylation site of serine 307 (T-E-SP-I-T). |
Genebank Info (Protein)
NP_005535 |
| |
|
|
Immunohistochemical analysis of IRS-1 (er307) in paraffin-embedded human breast carcinoma tissue using IMG-90304-1. |
|
|
|
|
|
| - |
 |
|
Reference
1. Karin, GS. et al. Biochemical and Biophysical Research Communications. 363:840-845 (2007).
2. Jodel, G. et al. Molecular Endocrinology. 21:2294-2302 (2007).
|
|
|
|
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
|
|
|
|
|
