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Peptide-affinity Purified Polyclonal Antibody to BRCA1 (Ser1423) |
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| Catalog No: |
IMG-90307-1 |
| Contents: |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. |
| Species: |
Human |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 1:50-1:100
Western Blot: 1:500-1:1000 |
| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
K562 cells |
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Background
The breast cancer tumor-suppressor gene, BRCA1, encodes a protein with a BRCT domain, a motif that is found in many proteins that are implicated in DNA damage response and in genome stability. It combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC for BRCA1-associated genome surveillance complex. BRCA1 functions in the nuclear processes of transcription, chromatin remodeling and silencing, and in various DNA repair mechanisms. It also has ubiquitin ligase activity. It regulates key effectors that control the G2/M checkpoint and is therefore involved in regulating the onset of mitosis. It is a protein of unknown structure and has a few identifiable features. The C-terminus of BRCA1 contains two 95-residue BRCT domains. Pathological role of the protein has been identified in breast and ovarian cancer. Many alternatively spliced transcript variants have been described for this gene with only some have had their full-length natures identified. |
Antigen
This antibody was generated by immunizing rabbits with a synthetic phosphopeptide corresponding to human BRCA1 around the phosphorylation site of serine 1423 (H-G-SP-Q-P). |
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Western blot analysis of BRCA1 (Ser1423) using IMG-90307-1 in K562 cells treated with UV (20min). |
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Immunohistochemical analysis of BRCA1 (1423) in paraffin-embedded human breast carcinoma tissue using IMG-90307-1. |
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Reference
1. Kashima, K. et al. Jpn. J. Canc. Res. 91:399-409 (2000).
2. Ronit, Y. et al. Nature Genetics. 30:285-289 (2002).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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