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Peptide-affinity Purified Polyclonal Antibody to CDC2 (Tyr15) |
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| Catalog No: |
IMG-90308-1 |
| Contents: |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. |
| Species: |
Human, Mouse, Rat |
| Host: |
Rabbit |
| Application: |
Western Blot: 1:500-1:1000 |
| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
COLO, HepG2 and K562 cells |
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Background
Cdc2 is a highly conserved protein serine kinase that plays a key role in regulation of the cell cycle. The ability of cdc2 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2. The protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Cdc2 during mitosis, signals the inactivation of major anabolic processes such as transcription, mRNA processing, translation, and ribosome biogenesis, thus providing energy needed for the radical and energetically costly structural reorganization of the cell. This is accomplished by phosphorylation and inactivation of several key anabolic elements, including TFIIIB, TFIID, RNA polymerase II, poly(A) polymerase, and translation elongation factor 1. Human Cdc2 gene is usually localized in the chromosomal region 10q21.1. |
Antigen
This antibody was generated by immunizing rabbits with a synthetic phosphopeptide corresponding to human CDC2 around the phosphorylation site of tyrosine 15 (G-T-YP-G-V) |
Genebank Info (Protein)
NP_001777 |
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Western blot analysis of CDC2 (Tyr15)using IMG-90308-1 in COLO (Lane 1) and HepG2 (Lane 2) and K562 (Lane 3 & 4) cells. |
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Reference
1. Jameel S. et al. J. Biol. Chem. 278:16433-16442 (2003).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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