|
|
|
| |
Peptide-affinity Purified Polyclonal Antibody to PKR (Thr451) |
|
|
| |
|
|
| |
|
| Catalog No: |
IMG-90337-1 |
| Contents: |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. |
| Species: |
Human, Mouse |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 1:50-1:100 |
| Storage: |
Store at -20°C. |
|
 |
|
|
|
|
|
|
Background
PKR (protein kinase, activatable by RNA) is a double-stranded (ds) RNA-activated protein kinase that plays a key role in the innate immunity response to viral infection in higher eukaryotes. PKR contains an N-terminal dsRNA-binding domain that consists of consensus dsRNA-binding motifs and a C-terminal kinase domain containing conserved motifs for protein kinase activity. PKR binds to dsRNA molecules during viral infection and triggers antiviral innate defense mechanisms including the induction of type I interferons and downregulation of gene expression. One of the most well characterized substrates of activated PKR is the eukaryotic tranlation initiation facter, eIF2. PKR phosphorylates eIF2 during virus infection, which ultimately leads to an inhibition in protein synthesis and block in viral replication. Human PKR maps to 2p22-p21 region of human chromosome. |
Antigen
This antibody was generated by immunizing rabbits with a synthetic phosphopeptide corresponding to human PKR around the phosphorylation site of threonine 451 (K-G-TP-L-R). |
Genebank Info (Protein)
NP_002750 |
| |
|
|
Immunohistochemical analysis of PKR (Thr451) in paraffin-embedded human colon carcinoma tissue using IMG-90337-1. |
|
|
|
|
|
| - |
 |
|
Reference
1. Langland, JO. et al. Virus Research. 119:100-110 (2006).
|
|
|
|
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
|
|
|
|
|
