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Peptide-affinity Purified Polyclonal Antibody to HDAC8 (Ab-39) |
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| Catalog No: |
IMG-90471-2 |
| Contents: |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. |
| Species: |
Human |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 1:50-1:100
Western Blot: 1:500-1:1000 |
| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
NIH/3T3 cells |
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Background
HDAC8 is a 377 residue class I enzyme that lies close to the phylogenetic boundary between the class I and class II histone deacetylases and maps to the X chromosome (Xq13). HDAC8, under the regulation of PKA, plays an important role in determining the acetylation state of histones. HDAC8 is phosphorylated by cyclic AMP-dependent protein kinase A (PKA) which decreases the activity of HDAC8 and leads to the hyperacetylation of histones H3 and H4. Pathological role of HDAC8 has been observed in one of the most frequent types of acute myeloid leukemia (AML). |
Antigen
This antibody was generated by immunizing rabbits with a synthetic non-phosphopeptide corresponding to human HDAC8 around the phosphorylation site of serine 39 (R-A-SP-M-V). |
| Genebank Info (Protein): NP_060956 |
| Gene ID: 55869 |
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Western blot analysis of HDAC8 (Ab-39) using IMG-90471-2 in NIH/3T3 cells. |
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Immunohistochemical analysis of HDAC8 (Ab-39) paraffin-embedded human lung carcinoma tissue using IMG-90471-2. |
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Reference
1. John, R. et al. Structure. 12:1325-1334 (2004).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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