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Product : Antibody >> Apoptosis , Cancer Antibodies , Chromatin >> AFX (Ab-197)
      
 

Peptide-affinity Purified Polyclonal Antibody to AFX (Ab-197)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-90483-2
AFX (Ab-197)Purified, Peptide Affinity
100 µg
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Catalog No: IMG-90483-2
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Isotype: Rabbit IgG
Clone: N/A
Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns.
Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC (paraffin): 1:50-1:100
Western Blot: 1:500-1:1000
Storage: Store at -20C.
Recommended
Positive Control:
293 cells
 

Background
AFX protein belongs to the forkhead protein family. It is highly homologous to the human FKHR protein, the gene of which is disrupted by the t(2;13)(q35;q14), a chromosome rearrangement characteristic of alveolar rhabdomyosarcomas. AFX contains three putative phosphorylation motif sites for protein kinase B (PKB), Thr32, Ser197, and Ser262, and it is proposed that phosphorylated AFX stays in the cytosol and is imported to the nucleus through dephosphorylation to induce target gene expression. Nuclear expression of AFX results in the suppression of various responses to hypoxia, including decreased vascular endothelial growth factor, glucose transporter 1, and erythropoietin expression. Human AFX gene is localized to chromosomal region Xq13.1.

Antigen
This antibody was generated by immunizing rabbits with a synthetic non-phosphopeptide corresponding to human AFX around the phosphorylation site of serine 197 (A-A-SP-M-D).

Genebank Info (Protein): NP_005929
Gene ID: 4303
SwissProt: P98177
 
AFX (Ab-197) Antibody

Western blot analysis of AFX (Ab-197) (Lanes 1 and 2) using IMG-90483-2 in 293 cells.

AFX (Ab-197) Antibody IMG-90483-2

Immunohistochemical analysis of AFX (Ab-197) in paraffin-embedded human lung carcinoma tissue using IMG-90483-2.

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Reference
1. Hidenori, M. et al. Journal of Biochemistry. 138:485-491 (2005).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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