|
|
|
| |
Peptide-affinity Purified Polyclonal Antibody to Bcr (Ab-177) |
|
|
| |
|
|
| |
|
| Catalog No: |
IMG-90509-2 |
| Contents: |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. |
| Species: |
Human, Mouse |
| Host: |
Rabbit |
| Application: |
Western Blot: 1:500-1:1000
|
| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
A431 cells |
|
 |
|
|
|
|
|
|
Background
Bcr was originally identified because of its fusion to Abl as a consequence of the Philadelphia chromosome translocation found in chronic myelogenous and acute lymphoblastic leukemias. It consists of multiple domains, including a C-terminal GTPase-activating domain for the small GTPase Rac, a DH, a PH and a C2 domain. The protein exists in two major forms. P210 contains the entire Bcr coding region except the domain encoding the GTPase-activating part of Bcr, whereas P190 includes only the amino-acid residues encoded by BCR exon 1. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Human Bcr protein is mapped to chromosomal region 22q11.23. |
Antigen
This antibody was generated by immunizing rabbits with a synthetic non-phosphopeptide corresponding to human Bcr around the phosphorylation site of tyrosine 177 (P-F-YP-V-N). |
| Genebank Info (Protein): NP_067585 |
| Gene ID: 613 |
| SwissProt: P11274 |
| |
|
Western blot analysis of Bcr (Ab-177) (Lanes 1 and 2) using IMG-90509-2 in untreated A431 cells, blocked or not blocked with immunizing peptide. |
|
|
|
|
|
| - |
 |
|
Reference
1. Suparna. M. et al. Oncogene. 22:8255–8262 (2003).
|
|
|
|
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
|
|
|
|
|
