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Product : Antibody >> Cell Cycle , Kinases & Phosphatase.. >> Bcr (Ab-177)
      
 

Peptide-affinity Purified Polyclonal Antibody to Bcr (Ab-177)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-90509-2
Bcr (Ab-177)Purified, Peptide Affinity
100 µg
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Catalog No: IMG-90509-2
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Isotype: Rabbit IgG
Clone: N/A
Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns.
Species: Human, Mouse
Host: Rabbit
Application: Western Blot: 1:500-1:1000
Storage: Store at -20C.
Recommended
Positive Control:
A431 cells
 

Background
Bcr was originally identified because of its fusion to Abl as a consequence of the Philadelphia chromosome translocation found in chronic myelogenous and acute lymphoblastic leukemias. It consists of multiple domains, including a C-terminal GTPase-activating domain for the small GTPase Rac, a DH, a PH and a C2 domain. The protein exists in two major forms. P210 contains the entire Bcr coding region except the domain encoding the GTPase-activating part of Bcr, whereas P190 includes only the amino-acid residues encoded by BCR exon 1. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Human Bcr protein is mapped to chromosomal region 22q11.23.

Antigen
This antibody was generated by immunizing rabbits with a synthetic non-phosphopeptide corresponding to human Bcr around the phosphorylation site of tyrosine 177 (P-F-YP-V-N).

Genebank Info (Protein): NP_067585
Gene ID: 613
SwissProt: P11274
 
Bcr (Ab-177) Antibody

Western blot analysis of Bcr (Ab-177) (Lanes 1 and 2) using IMG-90509-2 in untreated A431 cells, blocked or not blocked with immunizing peptide.

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Reference
1. Suparna. M. et al. Oncogene. 22:8255–8262 (2003).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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