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Product : Antibody >> Chromatin >> 4E-BP1 (Ab-45)
      
 

Peptide-affinity Purified Polyclonal Antibody to 4E-BP1 (Ab-45)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-90523-2
4E-BP1 (Ab-45)Purified, Peptide Affinity
100 µg
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Catalog No: IMG-90523-2
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Isotype: Rabbit IgG
Clone: N/A
Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns.
Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC (paraffin): 1:50-1:100
Western Blot: 1:500-1:1000
Storage: Store at -20C.
Recommended
Positive Control:
MDA-MB-435 cells
 

Background
The translation of mRNA in eukaryotic cells is regulated by the presence of amino acids through multiple mechanisms. One such mechanism involves the evolutionarily conserved serine/threonine kinase TOR (Target of rapamycin, also known as mTOR), which regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. One downstream target of TOR is the eukaryotic initiation factor 4E binding protein 1 (4E-BP1) whose phosphorylation prevents its association with eIF4E, preferentially stimulating translation of mRNAs containing long, highly structured 5-UTRs. Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation, at least in part by inhibiting the activity of TOR towards 4E-BP1.

Antigen
This antibody was generated by immunizing rabbits with a synthetic non-phosphopeptide corresponding to human 4E-BP1 around the phosphorylation site of threonine 45 (S-T-TP-P-G).

Genebank Info (Protein): NP_004086
Gene ID: 1978
SwissProt: Q13541
 
4E-BP1 (Ab-45) Antibody

Western blot analysis of 4E-BP1 (Ab-45) (Lanes 1 and 2) using IMG-90523-2 in MDA-MB-435 cells untreated or treated with EGF (200ng/ml, 5min).

4E-BP1 (Ab-45) Antibody IMG-90523-2

Immunohistochemical analysis of 4E-BP1 (Ab-45) in paraffin-embedded human breast carcinoma tissue using IMG-90523-2.

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Reference
1. Shamji, AF. et al. Mol. Cell. 12:271-280 (2003). 
2. Fingar, DC. et al. Oncogene. 23:3151-3171 (2004).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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