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Peptide-affinity Purified Polyclonal Antibody to Cortactin (Ab-421) |
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| Catalog No: |
IMG-90560-2 |
| Contents: |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. |
| Species: |
Human, Mouse |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 1:50-1:100 |
| Storage: |
Store at -20°C. |
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Background
Cortactin is a c-src substrate associated with sites of dynamic actin assembly at the leading edge of migrating cells promotes branched actin filament network formation by Arp2/3 complex. Cortactin is an F-actin-binding protein. The amino-terminal half of the protein is comprised of multiple copies of an internal, tandem 37- amino acid repeat while the carboxyl-terminal half contains a distal SH3 domain. Cortactin, present in several different cell types, is enriched in cortical structures such as membrane ruffles and lamellipodia. The properties of cortactin indicate its importance for microfilament-membrane interactions as well as transducing signals from the cell surface to the cytoskeleton. Human cortactin gene is localized on chromosome 11q13. |
Antigen
This antibody was generated by immunizing rabbits with a synthetic non-phosphopeptide corresponding to human Cortactin around the phosphorylation site of tyrosine 421 (P-I-YP-E-D). |
Genebank Info (Protein)
NP_031829 |
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Immunohistochemical analysis of Cortactin (Ab-421) in paraffin-embedded human breast carcinoma tissue using IMG-90560-2. |
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Reference
1. Wu, H. et al. The Journal of Cell Biology. 120:1417-1426 (1993).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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