|
|
|
| |
Peptide-affinity Purified Polyclonal Antibody to eIF2a (Ab-51) |
|
|
| |
|
|
| |
|
| Catalog No: |
IMG-90565-2 |
| Contents: |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. |
| Species: |
Human, Mouse, Rat |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 1:50-1:100
Western Blot: 1:500-1:1000
|
| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
K562 cells |
|
 |
|
|
|
|
|
|
Background
Eukaryotic initiation factor 2A (eIF2a) is a 65-kDa protein that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low [Mg2+]. eIF2a binds initiator tRNA in a GTP-independent manner, but require the presence of the small ribosomal subunit and the trinucleotide start codon, AUG. Phosphorylation of the protein terminates global protein translation and induces apoptosis. Human eIF2a gene is localized on chromosome 3q25.1. |
Antigen
This antibody was generated by immunizing rabbits with a synthetic non-phosphopeptide corresponding to human eIF2a around the phosphorylation site of serine 51 (E-L-SP-R-R). |
| Genebank Info (Protein): NP_114414 |
| Gene ID: 83939 |
| SwissProt: Q9BY44 |
| |
|
Western blot analysis of eIF2a (Ab-51) (Lanes 1 and 2) using IMG-90565-2 in K562 cells untreated or treated with IFN-a (100ng/ml, 20min). |
|
|
|
|
Immunohistochemical analysis of eIF2a (Ab-51) in paraffin-embedded human breast carcinoma tissue using IMG-90565-2. |
|
|
|
|
|
| - |
 |
|
Reference
1. Wendy, Zoll. et al. J. Biol. Chem. 277:37079-37087 (2002).
2. Chang, RCC. et al. Neuroreport. 13:2429-2432 (2002).
|
|
|
|
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
|
|
|
|
|
