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Peptide-affinity Purified Polyclonal Antibody to GSK3b (Ab-216) |
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| Catalog No: |
IMG-90581-2 |
| Contents: |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. |
| Species: |
Human, Mouse, Rat |
| Host: |
Rabbit |
| Application: |
Western Blot: 1:500-1:1000 |
| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
293 cells |
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Background
GSK3b is a proline directed serine/threonine kinase, originally identified as a result of its role in glycogen metabolism regulation. GSK3b is particularly abundant in the central nervous system, where it is neuron specific. It affects many fundamental cellular functions, including cell cycle, gene transcription, and cytoskeletal integrity. GSK3b has recently been implicated in many signaling pathways, such as Wnt and PI3/PKB signaling. Recently the pathological role of the protein has been identified in Alzheimer Disease (AD), a neurodegenerative disease. Human GSK3b has been mapped to chromosomal region 3q13.3. |
Antigen
This antibody was generated by immunizing rabbits with a synthetic non-phosphopeptide corresponding to human GSK3b around the phosphorylation site of tyrosine 216 (V-S-YP-I-C). |
Genebank Info (Protein)
NP_002084 |
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Western blot analysis of GSK3b (Ab-216) (Lanes 1 and 2) using IMG-90581-2 in 293 cells, untreated or treated with insulin (40?M, 30min). |
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Reference
1. Baolin, L. et al. Transgenic Research. 13:385-396 (2004).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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