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Polyclonal Antibody to CARMA1/ CARD11 |
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| Catalog No: |
IMX-5160 |
| Contents: |
100 ul of crude rabbit sera with 50% glycerol and 0.025% sodium azide |
| Isotype: |
Rabbit Ig |
| Clone: |
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| Purification: |
Crude rabbit sera |
| Species: |
Human |
| Predicted React: |
Mouse, Rat |
| Host: |
Rabbit |
| Application: |
Peptide ELISA: 1:100-1:1000 |
| Storage: |
Store at -20°C. |
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Background
Researchers have indicated that they want access to novel target antibodies quickly to aid in their research. IMGENEX is pleased to be able to offer researchers access to cutting-edge products that are still in the process of characterization/validation. IMGENEX’s ‘IMX’ antibody line is shown by ELISA to bind to the peptide used as immunogen. Investigators should empirically determine the suitability of the antibody, including optimal dilutions, for other applications of interest.
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We cannot guarantee that the yet-to-be-fully validated antibody will work in any application other than in an ELISA against the peptide used as immunogen and therefore cannot offer a refund if the antibody does not work in your application. |
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The antibody is offered at a lower price compared to a more highly validated antibody. |
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We are not able to provide the immunogen for the antibodies, but do provide the amino acid sequence. |
These antibodies are not fully validated and we appreciate your feedback. Customer feedback is used to help generate testing methodologies which can lead to further product validation or withdrawal.
CARMA1 is a 1154 amino acid protein playing a major role in the activation of NF-kappa-B through BCL10 and IKK. It contains a caspase-associated recruitment domain (CARD) and is thus included in the CARD protein family, a guanylate kinase-like domain and a PDZ (DHR) domain. The domain structure is similar to that of CARD14 protein and belongs to a class of proteins known as the membrane-associated guanylate kinase (MAGUK) family acting as molecular scaffolds. Binding of CARD11 and BCL10, a positive regulator of cell apoptosis and NF-kappaB activation, to each other occurs by CARD-CARD interaction and it stimulates the phosphorylation of BCL10. CARMA1 is detected in adult peripheral blood leukocytes, thymus, spleen and liver and in some cell lines except in HeLa S3, Molt-4, A549 and G431 cells. |
Antigen
Amino acids 236-252 (CKLERNQSLKLKNDIEN) of human CARMA1 protein were used as the immunogen. |
Genebank Info (Protein)
NP_115791 |
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Reference
1. Bertin,J., Wang,L., Guo,Y., Jacobson,M.D., Poyet,J.L., Srinivasula,S.M., Merriam,S., DiStefano,P.S. and Alnemri,E.S. CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF-kappa B J. Biol. Chem. 276 (15), 11877-11882 (2001)
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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