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Product : Antibody >> ELISA Antibodies , New Antibodies , Nuclear & Cytoplasmic Org.. , Transcription Factors >> BiP/GRP78
      
 

Polyclonal Antibody to BiP/GRP78
 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMX-5263
BiP/GRP78Sera
0.1 mL
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Catalog No: IMX-5263
Contents: 100 ul of crude rabbit sera with 50% glycerol and 0.025% sodium azide
Isotype: Rabbit Ig
Clone: N/A
Purification: Crude rabbit sera
Species: Human
Predicted React: Bovine, Chimpanzee, Chinese Hamster, Dog, Mouse, Rat
Host: Rabbit
Application: Peptide ELISA: 1:100-1:1000
Storage: Store at -20°C.
 

Background
Researchers have indicated that they want access to novel target antibodies quickly to aid in their research. IMGENEX is pleased to be able to offer researchers access to cutting-edge products that are still in the process of characterization/validation. IMGENEX’s ‘IMX’ antibody line is shown by ELISA to bind to the peptide used as immunogen. Investigators should empirically determine the suitability of the antibody, including optimal dilutions, for other applications of interest.

We cannot guarantee that the yet-to-be-fully validated antibody will work in any application other than in an ELISA against the peptide used as immunogen and therefore cannot offer a refund if the antibody does not work in your application.
The antibody is offered at a lower price compared to a more highly validated antibody.
We are not able to provide the immunogen for the antibodies, but do provide the amino acid sequence.


These antibodies are not fully validated and we appreciate your feedback. Customer feedback is used to help generate testing methodologies which can lead to further product validation or withdrawal.
  Principal Names: BiP; GRP78; Glucose-regulatory protein; HSPA5; Immunoglobulin heavy chain-binding protein; Endoplasmic reticulum luminal Ca2+ binding protein grp78; MIF2; Heat-shock 70KDa protein 5; Official Gene Symbol: HSPA5 Gene ID: 3309(Human) Gene Map Locus: 9q33-34.1(Human) GRP78, a 78KDa Glucose-regulated chaperone protein, is a member of Hsp70 family of heat shock proteins. It is a multifunctional protein consisting of an N-terminal ATPase domain and a C-terminal peptide-binding domain. It plays a vital role in ensuring Ig protein folding and maturation, maintaining permeability barrier of ER translocon during early stages of protein translocation, targeting misfolded proteins for proteasomal degradation, serving as a sensor for ER stress by regulating activation of ATF6, IRE1 and PERK and contributing to ER calcium stores. It regulates proper folding and maturation of other proteins including Prp, TSHR, apoB and LDL receptor. It translocates to the cell surface and binds to MHC class I. It also serves as a receptor for alpha2-macroglobulin-dependant signaling and for uptake of certain viruses. It might have a role as a protective factor against UVC-induced cell death.

Antigen
Amino acids 17-28 (RAEEEDKKEDVG) of human BiP protein were used as the immunogen.

Application Notes
The NCBI accession number for BiP is AAF42836.  The amino acid sequence used as immunogen is 100% homologous in a broad range of species.

Genebank Info (Protein)
NP_005338

Genebank Info (Nucleotide)
NP_005338

 
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Reference
1. Corrigall,V.M., Bodman-Smith,M.D., Brunst,M., Cornell,H. and Panayi,G.S., Inhibition of antigen-presenting cell function and stimulation of human peripheral blood mononuclear cells to express an antiinflammatory cytokine profile by the stress protein BiP: relevance to the treatment of inflammatory arthritis, Arthritis Rheum. 50 (4), 1164-1171 (2004).
2. Reddy,R.K., Mao,C., Baumeister,P., Austin,R.C., Kaufman,R.J. and Lee,A.S., Endoplasmic reticulum chaperone protein GRP78 protects cells from apoptosis induced by topoisomerase inhibitors: role of ATP binding site in suppression of caspase-7 activation, J. Biol. Chem. 278 (23), 20915-20924 (2003).
3. Watson,L.M., Chan,A.K., Berry,L.R., Li,J., Sood,S.K., Dickhout,J.G., Xu,L., Werstuck,G.H., Bajzar,L., Klamut,H.J. and Austin,R.C., Overexpression of the 78-kDa glucose-regulated protein/immunoglobulin-binding protein (GRP78/BiP) inhibits tissue factor procoagulant activity J. Biol. Chem. 278 (19), 17438-17447 (2003).
4. Shuda,M., Kondoh,N., Imazeki,N., Tanaka,K., Okada,T., Mori,K., Hada,A., Arai,M., Wakatsuki,T., Matsubara,O., Yamamoto,N. and Yamamoto,M., Activation of the ATF6, XBP1 and grp78 genes in human hepatocellular carcinoma: a possible involvement of the ER stress pathway in hepatocarcinogenesis, J. Hepatol. 38 (5), 605-614 (2003).
Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.

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All products are For Research Use Only, unless indicated otherwise. Not for use in diagnostic or therapeutic procedures.

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