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Product : Antibody >> New Antibodies , Nuclear & Cytoplasmic.. >> Histone H3
      
 

Polyclonal Antibody to Histone H3

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMX-6023
Histone H3Sera
0.1 mL
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Catalog No: IMX-6023
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ul of crude rabbit sera with 50% glycerol and 0.025% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit Ig
Species: Cow, Horse, Human
Host: Rabbit
Application: Peptide ELISA: 1:100-1:1000
Storage: Store at -20C.
 

Background
Researchers have indicated that they want access to novel target antibodies quickly to aid in their research. IMGENEX is pleased to be able to offer researchers access to cutting-edge products that are still in the process of characterization/validation. IMGENEX’s ‘IMX’ antibody line is shown by ELISA to bind to the peptide used as immunogen. Investigators should empirically determine the suitability of the antibody, including optimal dilutions, for other applications of interest.

We cannot guarantee that the yet-to-be-fully validated antibody will work in any application other than in an ELISA against the peptide used as immunogen and therefore cannot offer a refund if the antibody does not work in your application.
The antibody is offered at a lower price compared to a more highly validated antibody.
We are not able to provide the immunogen for the antibodies, but do provide the amino acid sequence.


These antibodies are not fully validated and we appreciate your feedback. Customer feedback is used to help generate testing methodologies which can lead to further product validation or withdrawal.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Histone H3 is of length 135 amino acid and is essential for the formation of the highly ordered chromatin structure that has been maintained throughout evolution. H3 histone is from the central building block of the nucleosomal core. It is wrapped around a nucleosome composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). Histone modifications and variants have key roles in the activation and silencing of genes. Phosphorylation of histone H3 at serine 10 and serine 28 is involved in transcriptional activation of genes responding to stress or mitogen-stimulated signaling pathways. Histones contribute to virtually all chromosomal processes, such as gene regulation, chromosome condensation, recombination, and replication.

Antigen
Amino acids 66-82 (LPFQRLVREIAQDFKTD) of human Histone H3 protein were used as the immunogen.

Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, horse and cow.

 
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Reference
1. Albig, W. et al. Hum. Genet. 97:486-491 (1996).
2. Albig, W. et al. Hum. Genet. 101:284-294 (1997).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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