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Polyclonal Antibody to DAZL |
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| Catalog No: |
IMX-6028 |
| Contents: |
100 ul of crude rabbit sera with 50% glycerol and 0.025% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Species: |
Chimpanzee, Cow, Horse, Human |
| Predicted React: |
Chicken, Dog, New World monkey, Mouse, Rat |
| Host: |
Rabbit |
| Application: |
Peptide ELISA: 1:100-1:1000 |
| Storage: |
Store at -20°C. |
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Background
Researchers have indicated that they want access to novel target antibodies quickly to aid in their research. IMGENEX is pleased to be able to offer researchers access to cutting-edge products that are still in the process of characterization/validation. IMGENEX’s ‘IMX’ antibody line is shown by ELISA to bind to the peptide used as immunogen. Investigators should empirically determine the suitability of the antibody, including optimal dilutions, for other applications of interest.
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We cannot guarantee that the yet-to-be-fully validated antibody will work in any application other than in an ELISA against the peptide used as immunogen and therefore cannot offer a refund if the antibody does not work in your application. |
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The antibody is offered at a lower price compared to a more highly validated antibody. |
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We are not able to provide the immunogen for the antibodies, but do provide the amino acid sequence. |
These antibodies are not fully validated and we appreciate your feedback. Customer feedback is used to help generate testing methodologies which can lead to further product validation or withdrawal.
DAZL is involved in mitosis and meiosis of human germ cells. The DAZL gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of diverse mammalian species. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In humans, DAZL is expressed in primordial germ cells, adult testis, and the ovary. It plays a central role during spermatogenesis. Defects in DAZL may cause infertility due to severe oligozoospermia (az) and non obstructive azoospermia. The concentrations of DAZL transcripts were found to be lower in men with spermatogenic failure. Drosophila and mouse mutants that have lost DAZL homologous gene function found to be azoospermic, just as with human azoospermia. |
Antigen
Amino acids 261-275 (NPENRLRNSVVTQDD) of human DAZL protein were used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, chimpanzee, horse and cow, 93% homologous in mouse, rat, dog, monkey and 73% homologous in chicken. |
Genebank Info (Protein)
NP_001342 |
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Reference
1. Kuo, PL. et.al Fertil. Steril. 81:1034-1040 (2004).
2. Brekhman, V. et al. Mol. Hum. Reprod. 6:465-468 (2000).
3. Teng, YN. et al. J. Clin. Endocr. Metab. 87:5258-5264 (2002).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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