100 ul of crude rabbit sera with 50% glycerol and 0.025% sodium azide. Sodium azide is highly toxic.
Peptide ELISA: 1:100-1:1000
Store at -20°C.
Background Researchers have indicated that they want access to novel target antibodies quickly to aid in their research. IMGENEX is pleased to be able to offer researchers access to cutting-edge products that are still in the process of characterization/validation. IMGENEX’s ‘IMX’ antibody line is shown by ELISA to bind to the peptide used as immunogen. Investigators should empirically determine the suitability of the antibody, including optimal dilutions, for other applications of interest.
We cannot guarantee that the yet-to-be-fully validated antibody will work in any application other than in an ELISA against the peptide used as immunogen and therefore cannot offer a refund if the antibody does not work in your application.
The antibody is offered at a lower price compared to a more highly validated antibody.
We are not able to provide the immunogen for the antibodies, but do provide the amino acid sequence.
These antibodies are not fully validated and we appreciate your feedback. Customer feedback is used to help generate testing methodologies which can lead to further product validation or withdrawal.
RNA polymerase II is a 113 amino acid protein belonging to RNA polymerase beta chain family. It contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. The C-terminal domain (CTD) of RNA polymerase II is required for efficient U2 snRNA transcription. Its localization identifies novel sites of transcription and provides insights into the regulation of transcription elongation. Ubiquitination of RNA polymerase II may have a role in the regulation of transcription-coupled DNA repair.
Amino acids 191-205 (DPEQLRQWMRSCVHR) of RNA polymerase II protein were used as the immunogen.
Reference 1.Yang, LY. et al. Int. J. Oncol. 22:683-9 (2003). 2.Sun, XJ. et al. J. Biol. Chem. 280:35261-71 (2005). 3.Jacobs, Ey. et al.Mol. Cell. Biol. 24:846-55 (2004).
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.