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Product : Antibody >> Apoptosis , New Antibodies >> APG5L
      
 

Polyclonal Antibody to APG5L

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMX-6091
APG5LSera
0.1 mL
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Catalog No: IMX-6091
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ul of crude rabbit sera with 50% glycerol and 0.025% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit Ig
Species: Cow, Dog, Human
Predicted React: Mouse, Rat
Host: Rabbit
Application: Peptide ELISA: 1:100-1:1000
Storage: Store at -20°C.
 

Background
Researchers have indicated that they want access to novel target antibodies quickly to aid in their research. IMGENEX is pleased to be able to offer researchers access to cutting-edge products that are still in the process of characterization/validation. IMGENEX’s ‘IMX’ antibody line is shown by ELISA to bind to the peptide used as immunogen. Investigators should empirically determine the suitability of the antibody, including optimal dilutions, for other applications of interest.

We cannot guarantee that the yet-to-be-fully validated antibody will work in any application other than in an ELISA against the peptide used as immunogen and therefore cannot offer a refund if the antibody does not work in your application.
The antibody is offered at a lower price compared to a more highly validated antibody.
We are not able to provide the immunogen for the antibodies, but do provide the amino acid sequence.


These antibodies are not fully validated and we appreciate your feedback. Customer feedback is used to help generate testing methodologies which can lead to further product validation or withdrawal.
ATG5L is a 275 amino acid protein belonging to the ATG5 family and is required for autophagy. It plays an important role in the apoptotic process within the modified cytoskeleton. It is known to function in autophagy and contributes to autophagic cell death by interacting with Fas-associated protein with death domain (FADD). It conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. Down-regulation of its expression in HeLa cells suppresses cell death and vacuole formation induced by IFN-gamma. It is highly expressed in apoptotic cells.

Antigen
Amino acid 167-183 (AINRKLMEYPAEENGFR) of human APG5L protein was used as the immunogen.

Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, cow and dog, 94% homologous in mouse and 88% homologous in rat.

Genebank Info (Protein): NP_004840
Gene ID: 9474
 
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Reference
1. Pyo, JO. et al.  J. Biol. Chem. 280:20722-20729 (2005).
2. Tanida, I. et al. J. Biol. Chem. 277:13739-13744 (2002).
3. Yung, HW. et al. EBS. Lett. 531:168-172 (2002).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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