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Polyclonal Antibody to DNER |
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| Catalog No: |
IMX-6154 |
| Contents: |
100 ul of crude rabbit sera with 50% glycerol and 0.025% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Species: |
Chimpanzee, Dog, Human, Mouse |
| Predicted React: |
Chicken |
| Host: |
Rabbit |
| Application: |
Peptide ELISA: 1:100-1:1000 |
| Storage: |
Store at -20°C. |
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Background
Researchers have indicated that they want access to novel target antibodies quickly to aid in their research. IMGENEX is pleased to be able to offer researchers access to cutting-edge products that are still in the process of characterization/validation. IMGENEX’s ‘IMX’ antibody line is shown by ELISA to bind to the peptide used as immunogen. Investigators should empirically determine the suitability of the antibody, including optimal dilutions, for other applications of interest.
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We cannot guarantee that the yet-to-be-fully validated antibody will work in any application other than in an ELISA against the peptide used as immunogen and therefore cannot offer a refund if the antibody does not work in your application. |
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The antibody is offered at a lower price compared to a more highly validated antibody. |
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We are not able to provide the immunogen for the antibodies, but do provide the amino acid sequence. |
These antibodies are not fully validated and we appreciate your feedback. Customer feedback is used to help generate testing methodologies which can lead to further product validation or withdrawal.
DNER, a neuron specific EGF-like repeat containing transmembrane protein, acts as a functional ligand of Notch and regulates morphologic differentiation of glia in the vertebrate brain. Like other Notch ligands, DNER specifically binds to Notch1 expressing cells and induces intracellular cleavage of Notch1 at the S3 site, thus mediating the Notch signaling pathway. The protein exerts a regulatory role in dendritic patterning of the central nervous system neurons. The human DNER gene is localized on chromosome 2q37. The protein is strongly expressed in several types of postmitotic neurons including cortical and hippocampal pyramidal neurons, cerebellar granule cells and Purkinje cells. |
Antigen
Amino acids 214-230 ([BLAST]RLVSFEVPQNTSVKIRQ[BLAST]) of human DNER protein were used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, chimpanzee, mouse and dog and 94% homologous in chicken. |
Genebank Info (Protein)
NP_620711 |
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Reference
1. Eiraku, M. et al. J. Biol. Chem. 277:25400-25407 (2002).
2. Nishizumi, H. et al. Neuroreport. 13:909-915 (2002).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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