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Polyclonal Antibody to APG12L |
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| Catalog No: |
IMX-6158 |
| Contents: |
100 ul of crude rabbit sera with 50% glycerol and 0.025% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Species: |
Chimpanzee, Dog, Human |
| Predicted React: |
Mouse, Rat, Cow, Chicken |
| Host: |
Rabbit |
| Application: |
Peptide ELISA: 1:100-1:1000 |
| Storage: |
Store at -20°C. |
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Background
Researchers have indicated that they want access to novel target antibodies quickly to aid in their research. IMGENEX is pleased to be able to offer researchers access to cutting-edge products that are still in the process of characterization/validation. IMGENEX’s ‘IMX’ antibody line is shown by ELISA to bind to the peptide used as immunogen. Investigators should empirically determine the suitability of the antibody, including optimal dilutions, for other applications of interest.
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We cannot guarantee that the yet-to-be-fully validated antibody will work in any application other than in an ELISA against the peptide used as immunogen and therefore cannot offer a refund if the antibody does not work in your application. |
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The antibody is offered at a lower price compared to a more highly validated antibody. |
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We are not able to provide the immunogen for the antibodies, but do provide the amino acid sequence. |
These antibodies are not fully validated and we appreciate your feedback. Customer feedback is used to help generate testing methodologies which can lead to further product validation or withdrawal.
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. APG12L is the human homologue of yeast APG12, a ubiquitin-activating enzyme E1-like protein essential for the conjugation system that mediates membrane fusion in autophagy. The protein possesses 27% identity and 48% similarity with the yeast APG12p. Northern blot analysis confirms the ubiquitous expression of the protein in human tissues. The gene encoding human APG12L protein is localized on chromosome 5q21-q22. |
Antigen
Amino acids 84-101 (SAAVSPGTEEPAGDTKKK) of human APG12L protein were used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, chimpanzee and dog, 94% homologous in mouse and cow, 88% homologous in rat and 77% homologous in chicken. |
Genebank Info (Protein)
NP_004698 |
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Reference
1. Noboru, M. et al. J. Biol. Chem. 273:33889-33892 (1998).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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