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Product : Antibody >> DNA Methylation & Rep.. , New Antibodies , Stem Cell Markers >> EZH2
      
 

Polyclonal Antibody to EZH2

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMX-6560
EZH2Sera
0.1 mL
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Catalog No: IMX-6560
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ul; crude rabbit sera with 50% glycerol and 0.025% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit Ig
Purification: Crude rabbit sera
Species: Human, New World monkey, Mouse, Rat
Host: Rabbit
Application: Peptide ELISA*: 1:100-1:1000
Storage: Store at -20°C.
 

Background
Researchers have indicated that they want access to novel target antibodies quickly to aid in their research. IMGENEX is pleased to be able to offer researchers access to cutting-edge products that are still in the process of characterization/validation. IMGENEX’s ‘IMX’ antibody line is shown by ELISA to bind to the peptide used as immunogen. Investigators should empirically determine the suitability of the antibody, including optimal dilutions, for other applications of interest.

We cannot guarantee that the yet-to-be-fully validated antibody will work in any application other than in an ELISA against the peptide used as immunogen and therefore cannot offer a refund if the antibody does not work in your application.
The antibody is offered at a lower price compared to a more highly validated antibody.
We are not able to provide the immunogen for the antibodies, but do provide the amino acid sequence.


These antibodies are not fully validated and we appreciate your feedback. Customer feedback is used to help generate testing methodologies which can lead to further product validation or withdrawal.
Enhancer of zeste homolog 2 is a polycomb group of protein that mediates repression of gene transcription at the chromatin level through its methyltransferase activity. It is a proliferative signal for multiple myeloma cells and has oncogenic activity in vivo, which is mediated by histone methyltransferase activity. EZH2 protein expression is induced by interleukin 6 (IL-6) in growth factor-dependent cell lines and is constitutive in IL-6-independent cell lines. Furthermore, EZH2 expression correlates with proliferation and B-cell terminal differentiation. Significantly, EZH2 protein inhibition by short interference RNA treatment results in MM cell growth arrest. Conversely, EZH2 ectopic overexpression induces growth factor independence. It has been found that the growth factor-independent proliferative phenotype in MM cell lines harboring a mutant N- or K-ras gene requires EZH2 activity. EZH2 has also been found to be a novel downstream target for the viral oncogenes in HPV-transformed cells and may serve as a therapeutic target for the treatment of cervical cancer.

Antigen
Amino acids 192-209 FPDKGTAEELKEKYKELT of human EZH2 protein were used as the immunogen.

Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, mouse, rat and monkey.

Genebank Info (Protein): NP_694543.1
Gene ID: 2146
SwissProt: Q15910
 
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Reference
1. Holland, D. et al. Cancer Res. 68:9964-9972 (2008).
2. Croonquist, PA. et al. Oncogene. 24:6269-6280 (2005).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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