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Polyclonal Antibody to HRS |
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| Catalog No: |
IMX-6587 |
| Contents: |
100 ul of crude rabbit sera with 50% glycerol and 0.025% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Purification: |
Crude rabbit sera |
| Species: |
Human, Mouse, Rat |
| Host: |
Rabbit |
| Application: |
Peptide ELISA*: 1:100-1:1000 |
| Storage: |
Store at -20°C. |
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Background
Researchers have indicated that they want access to novel target antibodies quickly to aid in their research. IMGENEX is pleased to be able to offer researchers access to cutting-edge products that are still in the process of characterization/validation. IMGENEX’s ‘IMX’ antibody line is shown by ELISA to bind to the peptide used as immunogen. Investigators should empirically determine the suitability of the antibody, including optimal dilutions, for other applications of interest.
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We cannot guarantee that the yet-to-be-fully validated antibody will work in any application other than in an ELISA against the peptide used as immunogen and therefore cannot offer a refund if the antibody does not work in your application. |
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The antibody is offered at a lower price compared to a more highly validated antibody. |
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We are not able to provide the immunogen for the antibodies, but do provide the amino acid sequence. |
These antibodies are not fully validated and we appreciate your feedback. Customer feedback is used to help generate testing methodologies which can lead to further product validation or withdrawal.
Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) is an early endosomal protein that plays a role in regulating the trafficking of growth factor-receptor complexes through early endosomes. It is involved in intracellular signal transduction mediated by cytokines and growth factors. It contains FYVE-type zinc finger, Ubiquitin-interacting motif (UIM) repeat and VHS domain. It suppresses DNA signaling upon stimulation by IL-2 and GM-CSF in association with STAM. HRS acts as a novel PELP1-binding protein providing evidence of a physiologic interaction between HRS and PELP1. The noted HRS-PELP1 interaction was accompanied by inhibition of the basal coactivator function of PELP1 upon estrogen receptor transactivation. HRS was found to sequester PELP1 in the cytoplasm, leading to the activation of MAPK in a manner that is dependent on the epidermal growth factor receptor but independent of the estrogen receptor, Shc and Src. |
Antigen
Amino acids 32-49 CDLIRQGDTQAKYAVNSI of human HRS protein were used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, mouse and rat. |
Genebank Info (Protein)
O14964 |
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Reference
1. Rayala, SK. et al. The Journal of Biological Chemistry. 281:4395-4403 (2006).
2. Yamashita, Y. et al. J. Cell Sci. 121:1727-1738 (2008).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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