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Goat Anti-Rabbit HRP Conjugate
Catalog No : 20301
Contents : 100 ul antibody in PBS with BSA (U.S. origin)
Isotype : Goat Ig
Purification : Immunoaffinity chromatography; absorbed for minimal cross-reactivity with human, mouse and rat
Species React : Rabbit
Host : Goat
Application
ELISA: 1:1000-1:20,000*
Western blot analysis: 1:1000-1:5,000*

Storage
Aliquot and store at -20°C.

Background
Secondary antibodies are antibodies that bind to primary antibodies or antibody fragments. They are typically labeled with probes that make them useful for detection, purification or cell sorting applications. Secondary antibodies may be polyclonal or monoclonal, and are available with specificity for whole Ig molecules or antibody fragments such as the Fc or Fab regions. Secondary antibodies are selected according to the source of the primary antibody, the class of the primary antibody (e.g., IgG or IgM), and the kind of label which is preferred. Identifying the optimal secondary antibody is normally done through trial and error method. Secondary antibodies are used in all types of immunoassays, most often in Western blot, immunohistochemistry, and immunocytochemistry, and occasionally in immunoprecipitation. Basic research, clinical analysis, and disease diagnosis also use secondary antibodies in ELISA and flow cytometry assays. They are also useful for cell sorting, fluorescence activated cell sorting, FACS.

Secondary antibodies are available in a variety of formats and conjugate types. These many options provide for excellent performance in many kinds of antibody-based detection and assay techniques.  When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.



Antigen
Rabbit Ig (H + L)

Application Notes
This product does not contain sodium azide. Sodium azide is an inhibitor of peroxidase.

Related Products
1. IMG-3073 [Peptide-affinity Purified Polyclonal Antibody to GAPDH - Loading Control]

Reference

Product Citations
1. Chondroitin-4-sulphate reduced oxidative injury in caerulein-induced pancreatitis in mice: the involvement of NF-kB translocation and apoptosis activation.  Campo G, A Anvenso, S Campo, G Nastasi, P Traina, A D'Ascola, A Calatroni. Experimental Biology and Medicine 233: 741-752 (2008). Imgenex antbodies cited:
1. Goat Anti-Rabbit HRP Conjugate (20301):  ELISA (mouse pancreactic tissue), Fig. 1B
2. Caspase-3 (IMG-144A): WB (mouse pancreatic tissue), Fig. 4B
3. Caspase-7 (IMG-5560): WB (mouse pancreatic tissue), Fig. 5B.

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Research purposes only. Not for diagnostic or in vivo use.