Secondary antibodies are antibodies that bind to primary antibodies or antibody fragments. They are typically labeled with probes that make them useful for detection, purification or cell sorting applications. Secondary antibodies may be polyclonal or monoclonal, and are available with specificity for whole Ig molecules or antibody fragments such as the Fc or Fab regions. Secondary antibodies are selected according to the source of the primary antibody, the class of the primary antibody (e.g., IgG or IgM), and the kind of label which is preferred. Identifying the optimal secondary antibody is normally done through trial and error method. Secondary antibodies are used in all types of immunoassays, most often in Western blot, immunohistochemistry, and immunocytochemistry, and occasionally in immunoprecipitation. Basic research, clinical analysis, and disease diagnosis also use secondary antibodies in ELISA and flow cytometry assays. They are also useful for cell sorting, fluorescence activated cell sorting, FACS.
Secondary antibodies are available in a variety of formats and conjugate types. These many options provide for excellent performance in many kinds of antibody-based detection and assay techniques. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. The antibody was isolated from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed. Flurophore/Protein: 12 mg/mg; 3.1 moles FITC per mole F(ab)2. Minimal cross-reaction to human, bovine, horse and rabbit.
1. Neutrophils express distinct RNA receptors in a non-canonical way. Berger M, C-Y Hsieh, M Bakele, V Marcos, N Riber, M Kormann, L Mays, L Hofer, O Neth, L Vitkov, WD Frautgartner, D von Schweinitz, R Kappler, A Hector, A Weber, D Hart. JBC 287:19409-19417 (2011). IMGENEX products cited:
1. TLR8-PE (IMG-321D): Flow (intracellular) + Flow (cell surface), Fig 1A (human neutrophils) & Fig 1B (HL60).2. TLR8 (IMG-321A): IF confocal microscopy, Fig 1C (human neutrophils).3. FITC-conjugated goat anti-rabbit secondary Ab (Cat no 20302), Flow (intracellular) + Flow (cell surface), various Figs.4. FITC-conjugated donkey anti-rat secondary Ab (Cat no 20204), Flow (intracellular) + Flow (cell surface), various Figs.
2. Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific. Wu J, Z Meng, M Jiang, E Zhang, M Trippler, R Broering, A Bucchi, F Krux, U Dittmer, D Yang, M Roggendorf, G Gerken, M Lu and JF Schlaak. Immunology 129(3):363-374; doi:10.1111/j.1365-2567.2009.03179.x (2009). IMGENEX products cited: TLR1 (IMG-5012), TLR2/CD282 (IMG-6320C), TLR3/CD283 (IMG-516), TLR4/CD284 (IMG-5031A), TLR5 (IMG-664), TLR6/CD286 (IMG-527), TLR7 (IMG-581A), TLR8/CD288 (IMG-321A), TLR9/CD289 (IMG-305A), Anti-Rabbit IgG (H+L) (20302), Anti-Mouse IgG (H+L) (20102), Rabbit IgG Isotype Control (20304), Mouse IgG1 Isotype Control (20109).