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Polyclonal Antibody to FENS1
Catalog No : IMG-3268
Contents : 0.1 mg of purified antibody.  0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Isotype : Goat Ig
Clone : N/A
Purification : Antigen Affinity Chromatography
Species React : Human
Predicted React : Mouse, Rat
Host : Rabbit
Application
Western blot analysis: 1µg/ml
IHC-P: 4-6µg/ml
Storage
Aliquot and store at -20°C.  Minimize freezing and thawing.

Background
Principal Names – WDFY1; WDF1; FENS-1; KIAA1435; WD repeat and FYVE domain containing 1; WD40 and FYVE domain containing 1; phosphoinositide-binding protein SR1 Official Gene Symbol - WDFY1 GenBank Accession Number – NP_065881; NP_848127 GeneID - 57590 (human)



Antigen
Peptide with sequence VVGCSLATGFSPH, from C Terminus of the protein sequence according to NP_065881; NP_848127.

Application Notes
Western blot: Preliminary experiments gave no signal but low background in Human Liver and HepG2 lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?IHC: In paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatoicytes. Recommended concentration, 4-6µg/ml.

IMG-3268 (4µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Reference
1. Ridley SH, Ktistakis N, Davidson K, Anderson KE, Manifava M, Ellson CD, Lipp P, Bootman M, Coadwell J, Nazarian A, Erdjument-Bromage H, Tempst P, Cooper MA, Thuring JW, Lim ZY, Holmes AB, Stephens LR, Hawkins PT. FENS-1 and DFCP1 are FYVE domain-containing proteins with distinct functions in the endosomal and Golgi compartments. J Cell Sci. 2001 Nov;114(Pt 22):3991-4000. PMID: 11739631
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