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| Peptide-affinity Purified Polyclonal Antibody to KPNA3/Karyopherin (Importin) alpha 4 |
| Catalog No |
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IMG-3568 |
| Contents |
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0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Isotype |
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Goat Ig |
| Clone |
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N/A |
| Purification |
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Antigen Affinity Chromatography |
| Species React |
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Human |
| Predicted React |
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Mouse |
| Host |
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Goat |
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| Application
Western blot analysis: 0.1-0.5µg/ml
IHC (paraffin): 5-10µg/ml
Storage
Store at -20°C.
Recommended Positive Control : Human Testis lysate |
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Background
Principal Names: KPNA3; SRP4; hSRP1; SRP1gamma; karyopherin alpha 3 (importin alpha 4); importin alpha 4. Official Symbol: KPNA3. GenBank Accession Number(s): NP_002258. GeneID: 3839 (human); 16648 (mouse). Gene Ontology Terms: nuclear localization sequence. binding; NLS-bearing substrate-nucleus import; protein complex assembly; importin, alpha-subunit; nuclear pore. |
Antigen
Peptide with sequence CDPTANLQTKEFNF, from the C Terminus of the protein sequence according to NP_002258.
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Application Notes
Peptide ELISA: antibody detection limit dilution >1/32,000. Western Blot: Approx 55-60kDa band observed in Human Testis and Kidney lysates (predicted size of approx. 58kDa according to NP_002258). Recommended for use at 0.1-0.5µg/mlImmunohistochemistry: In paraffin embedded Human Heart shows strong pixulate staining of myocardial fibres in transverse section. Recommended concentration, 5-10µg/ml. |
Genebank Info (Protein)
NP_002258
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Western blot analysis of KPNA3/Importin alpha 4 in Human Testis lysate using IMG-3568 at 0.1 ug/ ml. |
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IHC analysis of KPNA3/Karyopherin (Importin) alpha 4 in paraffin embedded Human Heart using IMG-3568 at 5µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
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Reference
1. Kohler M, Ansieau S, Prehn S, Leutz A, Haller H, Hartmann E.
Cloning of two novel human importin-alpha subunits and analysis of the expression pattern of the importin-alpha protein family.
FEBS Lett. 1997 Nov 3;417(1):104-8.
PMID: 9395085
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