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| Peptide-affinity Purified Polyclonal Antibody to UBA6 (UBE1L2, MOP-4) |
| Catalog No |
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IMG-6102A |
| Contents |
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100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype |
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Rabbit Ig |
| Purification |
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Peptide affinity chromatography was used to purify this antibody |
| Species React |
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Chimpanzee, Human, Mouse |
| Predicted React |
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Rat, Horse, Cow |
| Host |
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Rabbit |
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| Application
Western blot analysis: 1-3 ug/ml
IHC (p): use antibody at 10 ug/ml
Storage
Store UBA6 antibody at 4°C, stable for 6 months. For long term storage, aliquot and store antibody at -20°C.
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Background
UBA6 (UBE1L2, MOP-4) is a novel E1 enzyme specific for ubiquitin and belongs to the ubiquitin-activating E1 family. It is a part of the cascade to attach ubiquitin (Ub) covalently to substrate proteins. UBA6 activates ubiquitin by first adenylating its carboxy-terminal glycine residue with ATP and then linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. Its isoforms influence spermatogenesis and male fertility. UBA6 is highly expressed in human monocytes. |
Antigen
A portion of amino acids 300-350 of human UBA6 was used as the immunogen for this antibody.
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Application Notes
The amino acid sequence used as immunogen for this antibody is 100%
homologous in human (isoform CRA_a and CRA_b) and chimpanzee, 88% homologous in
horse and cow, 82% homologous in rat and 76% homologous in mouse. |
Genebank Info (Protein)
NP_060697
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Western blot analysis of human UBA6 in Daudi cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) RAW using UBA6 antibody at 2 ug/ml. |
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IHC analysis of UBA6 in human brain, cortex, using UBA6 antibody at 10 ug/ml. |
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Reference
1. Jin, J. et al. Nature. 447:1135-1138 (2007).
2. Pelzer, C. et al. J. Biol. Chem. 282:23010-23014 (2007).
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