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| Monoclonal Antibody to Qa-1b (Azide-free) |
| Catalog No |
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IMG-6223E |
| Contents |
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0.5 mgs in 1 ml PBS. Filter sterilized. Sodium azide free. |
| Isotype |
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Mouse IgG1, Kappa |
| Clone |
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6A8.6F10.1A6 |
| Purification |
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Protein G Chromatography |
| Species React |
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Mouse |
| Host |
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Mouse |
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| Application
Flow (Cell Surface): 0.1-0.5 ug/10^6 cells
Western blot analysis: 1-2 ug/ml
IP: reported
FA (Neutralization): reported
Storage
Aliquot and store frozen at -20°C.
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Background
Qa-1, a nonclassical class I histocompatibility molecule encoded by the T23 gene in the T region expressed in mice, predominantly assembles with a single nonameric peptide, Qdm, derived from the signal sequence of certain class Ia molecules. The Qa-1/Qdm complex serves as a ligand for CD94/NKG2 natural killer (NK) receptor(s) express on a major fraction of NK cells and CD8+ immuno-suppressive regulatory T cells. Qa-1, expressing on immuno-regulatory B cells and other antigen presenting cells, serves for antigen-presentation to immuno-regulatory CD8+ T. Moreover, Qa-1 serves for MHC restrictive function because, unlike conventional MHC molecules, it transiently expresses on activated CD4+ T cells. Recently it has also been shown that Qa-1 is ubiquitously expressed in the immune-privileged anterior chamber of the eye. Additionally, it has been suggested that TCR specific, Qa-1-restricted regulatory CD8+ T cells selectively down regulate antigen-activated T cells expressing TCRs of certain affinities. These regulatory CD8+ T cells may control the peripheral TCR repertoire during the course of immune responses to both self and foreign antigens. The 6A8.6F10.1A6 mAb can be used for flow cytometric assay to detect Qa-1b on immuno-regulatory antigen presenting cells and activated splenic T cells on Qa-1b expressing mice (e.g. BALB/c, C57BL/6) and it can also be used for functional assays to block target cell recognition by CTLs specific for Qa-1 restricted antigen presentation and other functional study where Qa-1b associated immuno-regulation may work. |
Antigen
Animals were immunized with a synthetic peptide from the alpha-2 domain of Qa-1b. Qa-1 is a Class Ib molecule recognized by cytotoxic T cells and natural killer cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells.The antibody reacts with the Qa-1b Class Ib molecule.
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Genebank Info (Protein)
NP_001129540
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Flow cytometric analysis of Qa-1b in unstimulated Balb/c mouse splenocytes using A) isotype control and B) IMG-6223A (with an anti-mouse IgG1-PE secondary) at 0.25 ug/10^6 cells. A shift is seen in only a small percentage of cells. |
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Flow cytometric analysis of Qa-1b in 72 hour ConA-stimulated Balb/c mouse splenocytes using A) isotype control and B) IMG-6223A (with an anti-mouse IgG1-PE secondary) at 0.25 ug/10^6 cells. A shift is seen in the entire population of cells. |
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Western blot analysis of Qa-1b in mouse thymus tissue lysate using IMG-6223A at 2 ug/ml. |
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Reference
1. Lu, L. et al. Immunol. Rev. 212:51-59 (2006).
2. Kim, CY. et al. Ocul. Immunol. Inflamm. 13:271-277 (2005).
3. Jiang, H. et al. Proc. Natl. Acad. Sci. U S A. 98:6301-6306 (2001).
4. D'Orazio, TJ. et al. J. Immunol. 166:26-32 (2001).
5. Jensen, PE. et al. Immunol. Res. 29:81-92 (2004).
6. Kraft, JR. et al. J. Exp. Med. 192:613-24 (2000).
7. Stanton, TH. et al. Immunogenetics. 3:525-531 (1976).
8. Jiang, H. et al. Annu. Rev. Immunol. 18:185-216 (2000).
9. Vance, RE. et al. J. Exp. Med. 188:1841-1848 (1998).
10. Noble, A. et al. J. Immunol. 160:566-571 (1998).
11. Suvas, S. et al. J. Immunol. 176:1703-1711 (2006).
12. Lo, WF. et al. J. Immunol. 162:5398-5406 (1999).
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