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| Monoclonal Antibody to CD3 (Clone OKT3) |
| Catalog No |
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IMG-6240A |
| Contents |
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100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype |
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Mouse IgG2a, Kappa |
| Clone |
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OKT3 |
| Purification |
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Protein G Chromatography |
| Species React |
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Human |
| Host |
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Mouse |
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| Application
Flow (Cell Surface): 0.1-0.25 ug/10^6 cells
Storage
Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C.
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Background
CD3 (T3), a complex T cell marker, is known to associate noncovalently with the a/b or g/z heterodimer of the T cell antigen receptor (TCR) to form the most complex transmembrane (TM) receptor structures. Structurally, CD3 consists of four distinct membrane glycoprotein isoforms (CD3g, CD3e, CD3s and the zeta chain) to generate activation signals. CD3 is specially engaged in antigen recognition and is known to play an important role in mediating signals that are critical for T cell development in the thymus, proliferation, and induction of T cell-mediated immune responses against infectious agents and also in the differentiation of T cells into effector and memory populations. CD3 usually expresses in the cytoplasm of prothymocytes, and on the surface of about 95% of thymocytes, but cytoplasmic CD3 is lost as the cells differentiate into medullary thymocytes. Apart from its role as an important marker in the classification of malignant lymphomas and lymphoid leukaemia, CD3 can also be useful for the identification of T cells in celiac disease, lymphocytic colitis and colorectal carcinomas associated with loss of a mismatch repair protein. CD3 indirectly plays an important role in immunomodulation whereas the anti-CD3antibody may be used in in vitro Treg assays to generate effector T cells. |
Antigen
Human PBMCs were used as immunogen.
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Cell surface flow analysis of CD3 in 10^6 human PBMC using IMG-6240A at 0.2ug. Green represents isotype control; red represents anti-CD3 antibody. |
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Related Products
1. View all human CD3 (clone OKT3) antibody sizes and formats here
View all mouse CD3 (clone 145-2C11) antibody sizes and formats here
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Reference
1. Evans G. A. et al. Immunogenetics 28: 365-373, (1988).
2. Brygida Bisikirska et al. J Clin Invest. 115: 2904–2913, (2005).
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Product Citations
1. An Epstein-Barr Virus (EBV) mutant with enhanced BZLF1 expression causes lymphomas with abortive lytic EBV infection in a humanized mouse model. Ma D S, X Yu, J E Mertz, J E Gumperz, E Reinheim, Y Zhou, W Tang, W J Burlingham, M L Gulley, S C Kenney. J Virol 86 7976-7987 (2012). IMGENEX product cited: CD3 (IMG-6240E). FA: Mice received 2 ug of CD3 antibody intravenously per day, starting 1 day after EBV infection, Fig 1A & Fig 1B. |
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