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| Monoclonal Antibody to human CD19 (PE Conjugate) |
| Catalog No |
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IMG-6321D |
| Contents |
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Antibody in 1 ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype |
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Mouse IgG1 |
| Clone |
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CB19 |
| Purification |
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Protein G Chromatography |
| Species React |
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Human |
| Host |
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Mouse |
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| Application
Flow (Cell Surface): 10 ul/10^6 cells (0.25 ug)
IF/ICC: reported in literature
IHC (frozen): reported in literature
Storage
Store at 4°C, stable for 6 months. DO NOT FREEZE; MATERIAL IS LIGHT-SENSITIVE.
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Background
CD19 is a member of the immunoglobulin superfamily with 556 amino acid and two immunoglobulin-like C2-type domains. As a cell surface protein, CD19 is known to form a complex with CD21, CD81 and CD225 in the membrane of mature B cells. A major function of CD19 is to assemble with the antigen receptor of B lymphocytes so as to decrease the threshold for antigen receptor dependent stimulation thus enhancing the specificity and sensitivity of B cells towards antigens. CD19 is an important protein for B cell antigen receptor signaling and regulation and also acts as a specialized adaptor protein for the amplification of Src family needed for this purpose. Regulation of growth of B cells is a major function of CD19 and its expression is confined to only B lymphocytes and follicular dendritic cells of the hematopoietic system. Increased expression of CD19 induces the production of autoantibody thus giving an insight to the regulatory role of CD19 in autoimmunity. Defects in CD19 are a cause of hypogammaglobulinemia. |
Antigen
Intact normal human B cell cells were used as immunogen to generate the CB19 clone. The exact CD19 epitope recognized by the antibody has not been mapped and is unknown.
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Cell surface flow cytometric analysis of CD19 in 1x106 human PBMC using 10 ul (0.25 ug) of IMG-6321D. Green represents isotype control (IMGENEX, 20106); red represents anti-CD19 antibody. Imgenex's cell surface staining flow assay kit (IMGENEX, 10084K) was used to test this product. Propidium iodide negative lymphocyte population gated for analysis. |
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Reference
1. Fujimoto, M. et al. Immunity. 13:47-57 (2000).
2. van Zelm, MC. et al. New Eng. J. Med. 354:1901-1912 (2006).
3. Carter, RH. et al. Science. 256:105-107 (1992).
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