Background
Interleukin-35 is a novel heterodimeric cytokine composed of the IL-12p35 subunit and the Epstein-Barr virus-induced gene 3 (EBI3) protein. As one of the IL-12 family members, Interleukin-35 is known to play an essential role in immune regulation of the CD4+CD25+ regulatory T (Treg) cells, from which Interleukin-35 is also specifically produced. Interleukin-35 is able to expand CD4+CD25+ Treg cells whereas it can inhibit proliferation of CD4+CD25- effector T (Teff) cells and development of proinflammatory Th17 cells, suppressing inflammatory responses.

Antigen
A full-length human Interleukin-35-Fc fusion protein was used as immunogen for this antibody.

Application Notes
The human Interleukin-35 protein is a chimeric protein consisting of the human IL-12a/p35 subunit (NP_000873.2) and the human EBI3 (NP_005746) protein.

Related Products
1. IMG-6766A [Monoclonal Antibody to IL-35 (Clone 15K8D10)]
2. View EBI3 antibodies
3. View IL-12a/p35 antibodies

Reference
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6. Yuya Takakubo, Yrjö T. Konttinen. Immune-Regulatory Mechanisms in Systemic Autoimmune and Rheumatic Diseases. Clin Dev Immunol. 2012; 2012: 941346.
7. Mingli Xu, Izuru Mizoguchi, Noriko Morishima, Yukino Chiba, Junichiro Mizuguchi, Takayuki Yoshimoto. Regulation of Antitumor Immune Responses by the IL-12 Family Cytokines, IL12, IL23, and IL27. Clin Dev Immunol. 2010; 2010: 832454.
8. Lauren W. Collison, Meenu R. Pillai, Vandana Chaturvedi, Dario A. A. Vignali. REGULATORY T CELL SUPPRESSION IS POTENTIATED BY TARGET T CELLS IN A CELL CONTACT, IL35- AND IL10-DEPENDENT MANNER. J Immunol. 2009 May 15; 182(10): 6121–6128.
9. Lauren W. Collison, Vandana Chaturvedi, Abigail L. Henderson, Paul R. Giacomin, Cliff Guy, Jaishree Bankoti, David Finkelstein, Karen Forbes, Creg J. Workman, Scott A. Brown, Jerold E. Rehg, Michael L. Jones, Hsiao-Tzu Ni, David Artis, Mary Jo Turk, Dario A. A. Vignali. Interleukin-35-mediated induction of a novel regulatory T cell population. Nat Immunol. 2010 December; 11(12): 1093–1101.
10. Mingli Xu, Izuru Mizoguchi, Noriko Morishima, Yukino Chiba, Junichiro Mizuguchi, Takayuki Yoshimoto. Regulation of Antitumor Immune Responses by the IL12 Family Cytokines, IL12, IL23, and IL27. Clin Dev Immunol. 2010; 2010: 832454.

Product Citations
1. Tumor-derived IL-35 promotes tumor growth by enhancing myeloid cell accumulation and angiogenesis.  Wang Zhihui, J-Q Liu, I Z Liu, R Shen, G Zhang, J Xu, S Basu, Y Feng, X-F Bai. JI 190:2415-2423 (2013). IHC (P), Fig 1: human large B cell lymphoma, nasopharyngeal carcinoma, skin, melanoma, lymph node metastatic melanoma.