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| Polyclonal Antibody to RNF31 (HOIP) |
| Catalog No |
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IMG-6768A |
| Contents |
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0.1 mg antibody in 0.2 ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype |
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Rabbit IgG |
| Purification |
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Protein A Chromatography |
| Species React |
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Human |
| Predicted React |
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Mouse, Rat, Chimpanzee, Rabbit, Frog, Orangutan, Opossum, Platypus |
| Host |
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Rabbit |
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Application
IHC (paraffin): 5 ug/ml
Storage
Store RNF31 (HOIP) antibody at 4oC, stable for 6 months. For long-term storage, aliquot and store antibody at -20oC.
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Background
RNF31, ring finger protein 31, is an E3 ubiquitin ligase also known as ZIBRA and HOIP is 110-120kDa. There are a number of functions attributed to RNF31 (HOIP) including zinc-ion binding and protein-poly ubiquitination (1-3). The NF-kB pathway is positively regulated by RNF31. T cell activation through T cell receptors is also modulated through RNF31. Based on its monoubiquitination of DAX-1, a mammalian orphan receptor associated with steroidogenesis, RNF31 (HOIP) also appears to be a requisite co-regulator of steroidogenic pathways (4). |
Antigen
A portion of amino acids 950-1000 from human RNF31 (HOIP) was used as the immunogen for this antibody.
Accession number: NP_060469.4
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Application Notes
The amino acid sequence used as immunogen for this antibody is 100% homologous in human, 94% in new world monkey, horse, naked mole rat, crab-eating macaque, rhesus monkey, white-cheeked gibbon, common chimpanzee, and sumatran orangutan, 88% in guinea pig, dog, common rabbit, and pig, 82% in panda, chinese hamster, african bush elephant, gray short-tailed opossum, platypus, rat, and clawed frog. |
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Immunohistochemical analysis of RNF31 (HOIP) using RNF31 antibody. Human rectum probed with RNF31 antibody at 5 ug/ml. |
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Reference
1. van der Reijden, BA et al 1999 TRIADS: a new class of proteins with a novel cysteine-rich signature. Protein Sci 8 1557-1561.
2. www.en.wikipedia.org
3. www.phosphosite.org
4. Ehrlund, A et al. 2009 E3 Ubiquitin ligase RNF31 cooperates with DAX-1 in transcriptional repression of steroidogenesis Mol. Cell. Biol. 29: 2230-2242.
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