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Polyclonal Antibody to SIRT1
Catalog No : IMG-6803A
Contents : 0.1 mg antibody in 0.2 ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype : Rabbit IgG
Purification : Protein A Chromatography
Species React : Cat, Chicken, Chimpanzee, Chinese Hamster, Cow, Dog, Frog, Horse, Human, Monkey, Mouse, Orangutan, Pig, Rabbit
Predicted React : Cichlid fish, Killifish, Zebrafish
Host : Rabbit
Application
Western blot analysis: 2-4 ug/ml

Storage
Store SIRT1 antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store antibody at -20°C.

Background
SIRT1 is a nuclear protein and member of the sirtuin protein family of deacetylating proteins.  SIRT1 is involved in regulating a number of cellular pathways including apoptosis and cell senescence, longevity and aging (1-3).  Not surprisingly, SIRT1 targets to forkhead (FOXO) transcription factors.  SIRT1 physically binds to the p65/RelA protein in the NF-kB complex and inhibits the transcriptional activity by deacetylating the p65 component (4).  SIRT1 anti-inflammatory responses have also been demonstrated  using resveratrol, a potent activator of SIRT1, which has been shown to potentiate the SIRT1-mediated anti-inflammatory response (5).  Detailed involvement of SIRT1 and NF-kB, and the control and interconnection of the Innate Immune and inflammatory response and aging, is reviewed by Salminen et al (6).



Antigen
A portion of amino acids 400-450 from human SIRT1 was used as the immunogen for this antibody.

Application Notes
The amino acid sequence used as immunogen for this antibody is 100% homologous in human, mouse, rat, orangutan, chimpanzee, panda, carolina anole, cow, new world monkey, dog, chinese hamster, horse, chicken, mole rat, rhesus monkey, wild turkey, opossum, platypus, rabbit, pig, zebra finch, frog, cat, and white-cheeked gibbon, and 89% homologous in zebrafish, cichlid fish, and killifish.



Genebank Info (Protein):
 NP_036370



Gene ID:
 23411

Reference
1. Guarente, L., 2000. Sir2 links chromatin silencing, metabolism, and aging. Genes Dev. 14, 1021–1026.
2. Vaziri, H., et al ., 2001. hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase. Cell 107, 149–159.
3. Brunet, A 2004 Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase. Science 303, 2011–2015.
4. Yeung, F., et al  2004 Modulation of NF-kappaB-dependent transcription and cell survival by the SIRT1 deacetylase. EMBO J. 23, 2369–2380.
5. Yang, S.-R., et al 2007 Sirtuin regulates cigarette smoke-induced proinflammatory mediator release via RelA/p65 NF-kappaB in macrophages in vitro and in rat lungs in vivo: implications for chronic inflammation and aging. Am. J. Physiol. Lung Cell Mol. Physiol. 292, L567–L576.
6. Salminen, A et al 2008  Activation of innate immunity system during aging: NF-kB signaling is the molecular culprit of inflamm-aging. Ageing Research Reviews 7: 83–105

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Research purposes only. Not for diagnostic or in vivo use.