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Polyclonal Antibody to Dnmt3L
Catalog No : IMG-6804A
Contents : 0.1 mg antibody in 0.2 ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype : Rabbit IgG
Purification : Protein A Chromatography was used to purify this antibody
Species React : Chimpanzee, Human
Predicted React : Chinese Hamster, Cow, Dog, Elephant, Horse, Macaque, Mole Rat, Mouse
Host : Rabbit
Application
IHC-P: use antibody at 5 ug/ml
Western blot analysis: 2-4 ug/ml

Storage
Store Dnmt3L antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store antibody at -20°C.

Background

DNMT3L is a nuclear DNA methyltransferase (DNMT)-like protein.  DNMTs are a family of enzymes that catalyze the transfer of a methyl group to DNA, a covalent genomic modification mechanism has a wide range of biological functions.   DNMTs are most well recognized for regulating and maintaining genomic imprinting, a germline-specific epigenetic genome process involving both DNA methylation and histone modifications. Imprinting results in certain genes being expressed in a parent-of-origin manner and is essential for normal development. 

Three active DNMTs have been identified in mammals, DNMT1, DNMT3A and DNMT3B.  However, DNMT3L lacks the amino acid residues necessary for methyltransferase activity and is catalytically inactive. Hence, DNMT3L  cannot directly methylate DNA.    Rather, DNMT3L is thought to function as a regulator of DNA methylation.  For example, DNMT3L co-localizes with DNMT3a and DNMT3b in the nucleus and stimulates their activity through protein-protein interactions possibly by anchoring the DNMTs to the chromatin. DNMT3L is thought to be required for maternal genomic imprints and there is evidence that DNMT3L also mediates transcriptional repression through interaction with histone deacetylase 1. Dysregulation of DNMT3L has been associated with cancer, and hence DNMT3L may play an important role in aberrant gene expression during carcinogenesis through alteration of methylation patterns.



Antigen
A portion of amino acids 130-180 from human Dnmt3L was used as the immunogen for this antibody.

Application Notes
The amino acid sequence used as immunogen for this antibody is 100% homologous in human, and chimpanzee, 94% in macaque, rhesus monkey, and white-cheeked gibbon, 89% in panda, cow, new world monkey, chinese hamster, horse, rat, and pig, 83% in dog, and mouse, 78% in naked mole rat, 73% in elephant, and 67% homologous in opossum.



Genebank Info (Protein):
 NP_037501.2



Gene ID:
 29947



SwissProt:
 Q9UJW3

Reference
1. Gokul G, G Ramakrishna, S Khosla. Reprogramming of HeLa cells upon DNMTL 3 overexpression mimics carcinogenesis. Epigenetics 4:322-329 (2009).
2. Wienholz BL, MS Kareta, AH Moarefi, CA Gordon, PA Ginno, F Chedin. DNMT3L modulates significant and distinct flanking sequence preference for DNA methylation by DNMT3A and DNMT3B in vivo.  PLoS Genet 6. E100106. Doi:10.1371/journal.pgen.1001106 (2010).

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Research purposes only. Not for diagnostic or in vivo use.