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Polyclonal Antibody to Dnmt3L
Catalog No : IMG-6805A
Contents : 0.1 mg antibody in 0.2 ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype : Rabbit IgG
Purification : Protein A Chromatography was used to purify this antibody
Species React : Chimpanzee, Human, White-cheeked gibbon
Predicted React : Chinese Hamster, Cow, Elephant, Horse, Macaque, Mole Rat, Mouse, New World monkey
Host : Rabbit
Application
IHC-P: use antibody at 5 ug/ml
Western blot analysis: 2-4 ug/ml

Storage
Store antibody at 4C, stable for 6 months. For long-term storage, aliquot and store antibody at -20C.

Background

DNMT3L is a nuclear DNA methyltransferase (DNMT)-like protein. DNMTs are a family of enzymes that catalyze the transfer of a methyl group to DNA, a covalent genomic modification mechanism has a wide range of biological functions. DNMTs are most well recognized for regulating and maintaining genomic imprinting, a germline-specific epigenetic genome process involving both DNA methylation and histone modifications. Imprinting results in certain genes being expressed in a parent-of-origin manner and is essential for normal development.

Three active DNMTs have been identified in mammals, DNMT1, DNMT3A and DNMT3B. However, DNMT3L lacks the amino acid residues necessary for methyltransferase activity and is catalytically inactive. Hence, DNMT3L cannot directly methylate DNA. Rather, DNMT3L is thought to function as a regulator of DNA methylation. For example, DNMT3L co-localizes with DNMT3a and DNMT3b in the nucleus and stimulates their activity through protein-protein interactions possibly by anchoring the DNMTs to the chromatin. DNMT3L is thought to be required for maternal genomic imprints and there is evidence that DNMT3L also mediates transcriptional repression through interaction with histone deacetylase 1. Dysregulation of DNMT3L has been associated with cancer, and hence DNMT3L may play an important role in aberrant gene expression during carcinogenesis through alteration of methylation patterns.



Antigen
A portion of amino acids 25-75 from human Dnmt3L was used as the immunogen for this antibody.

Application Notes
The amino acid sequence used as immunogen for this antibody is 100% homologous in human, chimpanzee, and white-cheeked gibbon, 95% in macaque, and rhesus monkey, 89% in panda, cow, new world monkey, and horse, 84% in pig, and opossum, 79% in naked mole rat, elephant, rat, and mouse, and 74% homologous in chinese hamster.



Genebank Info (Protein):
 NP_037501.2



Gene ID:
 29947



SwissProt:
 Q9UJW3

Reference
1. Gokul G, G Ramakrishna, S Khosla. Reprogramming of HeLa cells upon DNMTL 3 overexpression mimics carcinogenesis. Epigenetics 4:322-329 (2009).
2. Yuya Kobayashi, Devin M. Absher, Zulfiqar G. Gulzar, Sarah R. Young, Jesse K. McKenney, Donna M. Peehl, James D. Brooks, Richard M. Myers, Gavin Sherlock. DNA methylation profiling reveals novel biomarkers and important roles for DNA methyltransferases in prostate cancer. Genome Res. 2011 July; 21(7): 1017–1027.
3. Rachel Deplus, Carmen Brenner, Wendy A. Burgers, Pascale Putmans, Tony Kouzarides, Yvan de Launoit, François Fuks. Dnmt3L is a transcriptional repressor that recruits histone deacetylase. Nucleic Acids Res. 2002 September 1; 30(17): 3831–3838.
4. Hisato Kobayashi, Takayuki Sakurai, Misaki Imai, Nozomi Takahashi, Atsushi Fukuda, Obata Yayoi, Shun Sato, Kazuhiko Nakabayashi, Kenichiro Hata, Yusuke Sotomaru, Yutaka Suzuki, Tomohiro Kono. Contribution of Intragenic DNA Methylation in Mouse Gametic DNA Methylomes to Establish Oocyte-Specific Heritable Marks. PLoS Genet. 2012 January; 8(1): e1002440.
5. Kirsten M. Niles, Donovan Chan, Sophie La Salle, Christopher C. Oakes, Jacquetta M. Trasler. Critical Period of Nonpromoter DNA Methylation Acquisition during Prenatal Male Germ Cell Development. PLoS One. 2011; 6(9): e24156.
6. Bethany L. Wienholz, Michael S. Kareta, Amir H. Moarefi, Catherine A. Gordon, Paul A. Ginno, Frédéric Chédin. DNMT3L Modulates Significant and Distinct Flanking Sequence Preference for DNA Methylation by DNMT3A and DNMT3B In Vivo. PLoS Genet. 2010 September; 6(9): e1001106.
7. Natasha M. Zamudio, Hamish S. Scott, Katja Wolski, Chi-Yi Lo, Charity Law, Dillon Leong, Sarah A. Kinkel, Suyinn Chong, Damien Jolley, Gordon K. Smyth, David de Kretser, Emma Whitelaw, Moira K. O'Bryan. DNMT3L Is a Regulator of X Chromosome Compaction and Post-Meiotic Gene Transcription. PLoS One. 2011; 6(3): e18276.
8. K Almstrup, C E Hoei-Hansen, J E Nielsen, U Wirkner, W Ansorge, N E Skakkebæk, E Rajpert-De Meyts, H Leffers. Genome-wide gene expression profiling of testicular carcinoma in situ progression into overt tumours. Br J Cancer. 2005 May 23; 92(10): 1934–1941.
9. Wienholz BL, MS Kareta, AH Moarefi, CA Gordon, PA Ginno, F Chedin. DNMT3L modulates significant and distinct flanking sequence preference for DNA methylation by DNMT3A and DNMT3B in vivo.  PLoS Genet 6. E100106. Doi:10.1371/journal.pgen.1001106 (2010).

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Research purposes only. Not for diagnostic or in vivo use.