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Polyclonal Antibody to RBCK1
Catalog No : IMG-6807A
Contents : 0.1 mg antibody in 0.2 ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype : Rabbit IgG
Purification : Protein A Chromatography
Species React : Chimpanzee, Human, Mouse, Orangutan
Predicted React : Cow, Dog, Elephant, Macaque, Mole Rat, New World monkey
Host : Rabbit
Application
Western blot analysis: 2-4 ug/ml

Storage
Store RBCK1 antibody at 4oC, stable for 6 months. For long-term storage, aliquot and store antibody at -20oC.

Background
RBCK1, also known as HOIL-1L, acts as an E3 ubiquitin-protein ligase, or as part of an E3 complex that contains a zf-RanBP (Zn-finger in Ran binding protein) and a zf-C3HC4 (RING finger) domain. RBCK1 catalyzes the ubiquitination and degradation of IRF3. Both RBCK1 and IRF3 are activated by viral infections and IRF3 is a prerequisite trigger for induction of type I Interferons (1). RBCK1 also negatively regulates TNF and IL1 induced NF-B activation (2). Thus it appears RBCK1 and IRF3 interaction represents a controlling antiviral regulatory channel but also that RBCK1 and ubiquitination pathways (3, 4) also are related to NF-kB and its role as an anti-viral response pathway.



Antigen
A portion of amino acids150-200 from human RBCK1 was used as the immunogen for this antibody.

Application Notes
The amino acid sequence used as immunogen for this antibody is 100% homologous in human, orangutan, and chimpanzee, 94% in rhesus monkey, 89% in new world monkey, and macaque, 83% in cow, guinea pig, and mole rat, 78% in dog, elephant, and rabbit, 72% in panda, 67% in opossum, 61% in rat, and 55% homologous in mouse.



Genebank Info (Protein):
 NP_112506.2



Gene ID:
 10616



SwissProt:
 Q9BYM8

Reference
1. Zhang M et al 2008 Negative feedback regulation of cellular antiviral signaling by RBCK1 –mediated degradation of IRF3. Cell research 18: 1096-1104.
2. Tian Y,  et al. 2007 RBCK1 negatively regulates tumor necrosis factor- and interleukin-1-triggered NF-kappaB activation by targeting TAB2/3 for degradation. J Biol Chem 282:16776–16782.
3. Bayle J, et al  2006 The E3 ubiquitin ligase HOIL-1 induces the polyubiquitination and degradation of SOCS6 associated proteins. FEBS Lett 2006; 580:2609–2614.
4. Fitzgerald, KA et al 2003 IKK and TBK1 are essential components of the IRF3 signaling pathways Nature Immunol. 4: 491-496.

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Research purposes only. Not for diagnostic or in vivo use.