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| Polyclonal Antibody to RBCK1 |
| Catalog No |
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IMG-6807A |
| Contents |
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0.1 mg antibody in 0.2 ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype |
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Rabbit IgG |
| Purification |
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Protein A Chromatography |
| Species React |
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Chimpanzee, Human, Mouse, Orangutan |
| Predicted React |
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Cow, Dog, Elephant, Macaque, Mole Rat, New World monkey |
| Host |
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Rabbit |
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| Application
Western blot analysis: 2-4 ug/ml
Storage
Store RBCK1 antibody at 4oC, stable for 6 months. For long-term storage, aliquot and store antibody at -20oC.
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Background
RBCK1, also known as HOIL-1L, acts as an E3 ubiquitin-protein ligase, or as part of an E3 complex that contains a zf-RanBP (Zn-finger in Ran binding protein) and a zf-C3HC4 (RING finger) domain. RBCK1 catalyzes the ubiquitination and degradation of IRF3. Both RBCK1 and IRF3 are activated by viral infections and IRF3 is a prerequisite trigger for induction of type I Interferons (1). RBCK1 also negatively regulates TNF and IL1 induced NF-ŪB activation (2). Thus it appears RBCK1 and IRF3 interaction represents a controlling antiviral regulatory channel but also that RBCK1 and ubiquitination pathways (3, 4) also are related to NF-kB and its role as an anti-viral response pathway. |
Antigen
A portion of amino acids 150-200 from human RBCK1 was used as the immunogen for this antibody.
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Application Notes
The amino acid sequence used as immunogen for this antibody is 100% homologous in human, orangutan, and chimpanzee, 94% in rhesus monkey, 89% in new world monkey, and macaque, 83% in cow, guinea pig, and mole rat, 78% in dog, elephant, and rabbit, 72% in panda, 67% in opossum, 61% in rat, and 55% homologous in mouse. |
Genebank Info (Protein)
NP_112506.2
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Western blot analysis of RBCK1 using RBCK1 antibody. A549 cell lysate in the 1) pre immune sera and 2) abesence of immunizing peptide and 3) 3T3 cell lysate probed with 2 ug/ml of RBCK1 antibody. IMGENEX”¦s goat anti-rabbit Ig HRP secondary antibody (20301) and PicoTect ECL substrate solution (10087K) were used for this test. |
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Reference
1. Zhang M et al 2008 Negative feedback regulation of cellular antiviral signaling by RBCK1 –mediated degradation of IRF3. Cell research 18: 1096-1104.
2. Tian Y, et al. 2007 RBCK1 negatively regulates tumor necrosis factor- and interleukin-1-triggered NF-kappaB activation by targeting TAB2/3 for degradation. J Biol Chem 282:16776–16782.
3. Bayle J, et al 2006 The E3 ubiquitin ligase HOIL-1 induces the polyubiquitination and degradation of SOCS6 associated proteins. FEBS Lett 2006; 580:2609–2614.
4. Fitzgerald, KA et al 2003 IKKÕ and TBK1 are essential components of the IRF3 signaling pathways Nature Immunol. 4: 491-496.
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